Metabolomics unravel differences between Cameroon Dura and Deli Dura oil palm (Elaeis guineensis Jacq.) genetic backgrounds against basal stem rot

Mohd Din Amiruddin, . and Idris Abu Seman, . and Abrizah Othman, . and Nurazah Zain, . and Ahmad Kushairi, . and Umi Salamah Ramli, . Metabolomics unravel differences between Cameroon Dura and Deli Dura oil palm (Elaeis guineensis Jacq.) genetic backgrounds against basal stem rot. pp. 227-241. ISSN 1511-2780

Metabolomics is emerging as a powerful tool for screening of metabolites in both phenotyping and diagnostic analyses in plants. Metabolomics coupled with multivariate statistical analysis has provided a fast and unbiased comparative investigation of the metabolite composition in oil palm root. Basal stem rot (BSR) caused by the white rot fungus Ganoderma boninense is a common oil palm disease in south-east Asia. Little is known about the disease mechanism and there is yet no satisfactory treatment for it. Besides agronomic practice of reducing the disease inoculum in the field breeding for resistance to the disease is probably the most practical control. This study investigated metabolomic differences in oil palm planting materials with varying susceptibility to G. boninense from Cameroon and Deli genetic backgrounds. Liquid chromatography-quadrupole/ time-of-flight-mass spectrometry (LC-Q/TOF-MS) data was subjected to multivariate statistical analysis and discovered that the palms could be categorised into two clusters linked to Cameroon and Deli oil palms. Changes in metabolites involved in phenylpropanoid pathway and primary metabolism including shikimic acid glucose and malic acid were observed. The comprehensive and unbiased strategy for metabolite data analysis presented here can be adopted to screen wider oil palm germplasm for those potentially useful for breeding BSR-resistant palms.

Full text available from: Official URL: http://jopr.mpob.gov.my/metabolomics-unravel-diffe...

Abstract

Metabolomics is emerging as a powerful tool for screening of metabolites in both phenotyping and diagnostic analyses in plants. Metabolomics coupled with multivariate statistical analysis has provided a fast and unbiased comparative investigation of the metabolite composition in oil palm root. Basal stem rot (BSR) caused by the white rot fungus Ganoderma boninense is a common oil palm disease in south-east Asia. Little is known about the disease mechanism and there is yet no satisfactory treatment for it. Besides agronomic practice of reducing the disease inoculum in the field breeding for resistance to the disease is probably the most practical control. This study investigated metabolomic differences in oil palm planting materials with varying susceptibility to G. boninense from Cameroon and Deli genetic backgrounds. Liquid chromatography-quadrupole/ time-of-flight-mass spectrometry (LC-Q/TOF-MS) data was subjected to multivariate statistical analysis and discovered that the palms could be categorised into two clusters linked to Cameroon and Deli oil palms. Changes in metabolites involved in phenylpropanoid pathway and primary metabolism including shikimic acid glucose and malic acid were observed. The comprehensive and unbiased strategy for metabolite data analysis presented here can be adopted to screen wider oil palm germplasm for those potentially useful for breeding BSR-resistant palms.

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Item Type:	Article
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palms
AGROVOC Term:	Ganoderma
AGROVOC Term:	Metabolites
AGROVOC Term:	Disease resistance
AGROVOC Term:	Fungal diseases
AGROVOC Term:	Germplasm
AGROVOC Term:	Genetic resistance
AGROVOC Term:	Grinding
AGROVOC Term:	Extraction
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/24164

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