Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea

Amirah Nor Kamarudin, . and Idris Abu Seman, . and Zetty Norhana Balia Yusof, . Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea. pp. 218-226. ISSN 1511-2780

Thiamine plays an indispensable role as a cofactor in many metabolic reactions in all living organisms. The active form thiamine pyrophosphate (TPP) functions as a cofactor in important metabolic reactions including glycolysis pentose phosphate pathway and the tricarboxylic acid cycle. Besides that thiamine is also associated with the induction of systemic acquired resistance (SAR) in plants. Hendersonia toruloidea is an endophytic fungus originally isolated from oil palm roots which have been shown to have biocontrol activity. Seven months old oil palm seedlings were inoculated with H. toruloidea and the response on the expression of thiamine biosynthesis genes were investigated. Microscopy analysis was performed to visualise the colonisation of the fungus. Ribonucleic acid (RNA) was extracted from oil palm leaves at Day 1 7 15 and 30 post-treatment. Quantitative Real-time PCR (qRT-PCR) was performed to measure the level of expression of four key thiamine biosynthesis genes namely THI4 THIC TH1 and TPK. The results showed up to 12-fold increase in the expression of all gene transcripts at Day 1 and 7 post-treatment. This work provides first evidence of enhancement of thiamine biosynthesis by endophytic colonisation in oil palm seedlings.

Full text available from: Official URL: http://jopr.mpob.gov.my/thiamine-biosynthesis-gene...

Abstract

Thiamine plays an indispensable role as a cofactor in many metabolic reactions in all living organisms. The active form thiamine pyrophosphate (TPP) functions as a cofactor in important metabolic reactions including glycolysis pentose phosphate pathway and the tricarboxylic acid cycle. Besides that thiamine is also associated with the induction of systemic acquired resistance (SAR) in plants. Hendersonia toruloidea is an endophytic fungus originally isolated from oil palm roots which have been shown to have biocontrol activity. Seven months old oil palm seedlings were inoculated with H. toruloidea and the response on the expression of thiamine biosynthesis genes were investigated. Microscopy analysis was performed to visualise the colonisation of the fungus. Ribonucleic acid (RNA) was extracted from oil palm leaves at Day 1 7 15 and 30 post-treatment. Quantitative Real-time PCR (qRT-PCR) was performed to measure the level of expression of four key thiamine biosynthesis genes namely THI4 THIC TH1 and TPK. The results showed up to 12-fold increase in the expression of all gene transcripts at Day 1 and 7 post-treatment. This work provides first evidence of enhancement of thiamine biosynthesis by endophytic colonisation in oil palm seedlings.

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Item Type:	Article
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palms
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Gene expression
AGROVOC Term:	Genes
AGROVOC Term:	Biological control agents
AGROVOC Term:	Endophytes
AGROVOC Term:	Natural enemies
AGROVOC Term:	Seedlings
AGROVOC Term:	morphology
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 06:54
URI:	http://webagris.upm.edu.my/id/eprint/24169

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