Analysis of gene expression by est from SSH library in dwarf oil palm

Sharifah Shahrul Rabiah Syed Alwee, . and Parameswari Namasivayam, . and Mohamad Arif Abd Manaf, . and Intan Nur Ainni Mohamed Azni, . and Ho Chai Ling, . Analysis of gene expression by est from SSH library in dwarf oil palm. pp. 197-217. ISSN 1511-2780

Six subtracted cDNA libraries were constructed from PS1 and AG1 oil palm breeding lines using Suppression Subtractive Hybridisation (SSH) method. Six putative clones identified to be associated in dwarfism encodes for brassinosteroid biosynthesis-like protein (DWF1) brassinosteroid insensitive 1-associated receptor kinase 1 precursor (BRI1) late elongated hypocotyl protein (LHY) gibberellin receptor (GID1) sterol 24-methyltransferase 1 (SMT1) and E3 ubiquitin-protein ligase (E3Ub) were analysed. These candidate dwarfing genes are involved in various stages of brassinosteroids (BR) gibberellins (GA) biosynthesis and signaling pathways for plants growth and development. The expression levels of candidate genes were validated by quantitative Real-time PCR (qRT-PCR) by two reference genes manganese superoxide dismutase (PD569) and predicted protein IFH-1 like (EA1332). Higher expression level of BRI1 LHY and SMT1 genes were observed in dwarf palms compared to standard palms with normalised fold-difference of 2.3285 1.5620 and 4.9044 respectively. Statistical analysis of all potential transcripts showed further evidence of SMT1 as a potential molecular marker for the screening of dwarf palm planting materials with highly significant expression levels in dwarf palms compared to standard palms; however the expression levels of BRI and LHY genes were statistically insignificant.

Full text available from: Official URL: http://jopr.mpob.gov.my/analysis-of-gene-expressio...

Abstract

Six subtracted cDNA libraries were constructed from PS1 and AG1 oil palm breeding lines using Suppression Subtractive Hybridisation (SSH) method. Six putative clones identified to be associated in dwarfism encodes for brassinosteroid biosynthesis-like protein (DWF1) brassinosteroid insensitive 1-associated receptor kinase 1 precursor (BRI1) late elongated hypocotyl protein (LHY) gibberellin receptor (GID1) sterol 24-methyltransferase 1 (SMT1) and E3 ubiquitin-protein ligase (E3Ub) were analysed. These candidate dwarfing genes are involved in various stages of brassinosteroids (BR) gibberellins (GA) biosynthesis and signaling pathways for plants growth and development. The expression levels of candidate genes were validated by quantitative Real-time PCR (qRT-PCR) by two reference genes manganese superoxide dismutase (PD569) and predicted protein IFH-1 like (EA1332). Higher expression level of BRI1 LHY and SMT1 genes were observed in dwarf palms compared to standard palms with normalised fold-difference of 2.3285 1.5620 and 4.9044 respectively. Statistical analysis of all potential transcripts showed further evidence of SMT1 as a potential molecular marker for the screening of dwarf palm planting materials with highly significant expression levels in dwarf palms compared to standard palms; however the expression levels of BRI and LHY genes were statistically insignificant.

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Item Type:	Article
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palms
AGROVOC Term:	Dwarfism
AGROVOC Term:	Gene expression
AGROVOC Term:	Genes
AGROVOC Term:	RNA
AGROVOC Term:	Extraction
AGROVOC Term:	Isolation
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Gel electrophoresis
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 06:54
URI:	http://webagris.upm.edu.my/id/eprint/24175

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