In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab Scylla olivacea from Setiu Wetlands Terengganu Malaysia

Shumpei Ieahata, . and Nurul Aqilah Iberahim, . and Sharifah Rahmah Syed Muhammad, . and Alia Syafiqah Aznan, . and Khairulbariyyah Zakaria, . and Nadirah Musa, . and Muhd Danish Daniel Abdullah, . and Wan Nurhafizah Wan Ibrahim, . and Najiah Musa, . and Sharifah Noor Emilia Syed Jamil Fadaak, . and Lee Kok Leong, . and Nur Azna Saari, . and Yeong Yik Sung, . and Laith Abdul Razzak, . and Mohamed Tajuddin Abdul Manaf, . and Sandra Catherine Zainathan, . and Nor Hazwani Hamzah, . and Mazlan Abd Ghaffar, . In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab Scylla olivacea from Setiu Wetlands Terengganu Malaysia. pp. 62-74. ISSN 1823-8556

Mud crabs are among the commercially important aquatic animals because of high demand in Asian countries. Currently one of the main constraints for expansion of mud crab aquaculture is the inconsistent and low hatchery production of crab juveniles. The use of beneficial bacteria offers a promising approach in aquaculture to prevent infections by pathogenic bacteria and has been shown to help reduce mortality after infection. The goals of this study were to isolate identify and evaluate potential probiotic bacteria from the gut of wild orange mud crab from Setiu Wetlands Terengganu. Isolate was identified using BBLTM Crystal Gram Positive (GP) Identification System. Probiotic properties were evaluated including antibiotic susceptibility tolerances to pH bile salt trypsin and pepsin autoaggregation and coaggregation with Vibrio pathogens. Isolate UMTK003 was identified as Bacillus licheniformis. The isolate was able to withstand pH 4.5 to 9.5. Bile salt pepsin and trypsin tolerances were 0.1-0.4 0.1-0.7 and 0.4-1 respectively. Antibiotic susceptibility profiling showed multiple antibiotic resistance (MAR) index of 0.15. Autoaggregation was 79.830.0015. Coaggregation varied from 57.710.0050 (Vibrioharveyi) 52.410.0011 (Vibrio metschnikovii) 50.070.0011 (Vibrio cholera) to 43.250.0010 (Vibrio parahaemolyticus). These results suggested probiotic potential of B. licheniformis strain UMTK003 for mud crab aquaculture in Malaysia.

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Abstract

Mud crabs are among the commercially important aquatic animals because of high demand in Asian countries. Currently one of the main constraints for expansion of mud crab aquaculture is the inconsistent and low hatchery production of crab juveniles. The use of beneficial bacteria offers a promising approach in aquaculture to prevent infections by pathogenic bacteria and has been shown to help reduce mortality after infection. The goals of this study were to isolate identify and evaluate potential probiotic bacteria from the gut of wild orange mud crab from Setiu Wetlands Terengganu. Isolate was identified using BBLTM Crystal Gram Positive (GP) Identification System. Probiotic properties were evaluated including antibiotic susceptibility tolerances to pH bile salt trypsin and pepsin autoaggregation and coaggregation with Vibrio pathogens. Isolate UMTK003 was identified as Bacillus licheniformis. The isolate was able to withstand pH 4.5 to 9.5. Bile salt pepsin and trypsin tolerances were 0.1-0.4 0.1-0.7 and 0.4-1 respectively. Antibiotic susceptibility profiling showed multiple antibiotic resistance (MAR) index of 0.15. Autoaggregation was 79.830.0015. Coaggregation varied from 57.710.0050 (Vibrioharveyi) 52.410.0011 (Vibrio metschnikovii) 50.070.0011 (Vibrio cholera) to 43.250.0010 (Vibrio parahaemolyticus). These results suggested probiotic potential of B. licheniformis strain UMTK003 for mud crab aquaculture in Malaysia.

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Item Type:	Article
AGROVOC Term:	Scylla olivacea
AGROVOC Term:	Probiotics
AGROVOC Term:	Antibacterial agents
AGROVOC Term:	Aquatic animals
AGROVOC Term:	Aquatic organisms
AGROVOC Term:	In vitro
AGROVOC Term:	Evaluation
AGROVOC Term:	Sampling
AGROVOC Term:	Dry season
AGROVOC Term:	Centrifugation
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 07:08
URI:	http://webagris.upm.edu.my/id/eprint/24209

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