Fermentation of biodiesel-derived waste for 1 3-propanediol production with response surface methodology

Stasha Eleanor Rosland Abel, . and Loh Soh Keong, . Fermentation of biodiesel-derived waste for 1 3-propanediol production with response surface methodology. pp. 74-80.

The present study aimed to investigate the fermentation conditions favouring the maximum production of 1 3-propanediol (1 3-PD) from crude glycerol. Response surface methodology (RSM) based on central composite design (CCD) was applied in designing the experiments to evaluate the interactive effects of glycerol concentration (20 to 50 g litre-1) pH (6 to 8) temperature (30C to 40C) and incubation time (48 to 72 hr) on 1 3-PD production. A total of 30 experimental runs consisting of 16 factorial points eight axial points and six centre points were conducted. It was found that the derived optimum conditions were: 39.9 g litre-1 glycerol; pH: 7.6 temperature: 33C and incubation time: 59.1 hr. Under these conditions the 1 3-PD produced (9.85 g litre-1) was 2 higher than the predicted value by RSM (9.69 g litre-1) hence the experimental design employed in validating the results obtained was significant. The analysis of variance (ANOVA) showed high coefficient of the determination values (R2) of 0.9444. The fermentation using RSM was able to increase the 1 3-PD production by two-fold.

Full text available from: Official URL: http://jopr.mpob.gov.my/fermentation-of-biodiesel-...

Abstract

The present study aimed to investigate the fermentation conditions favouring the maximum production of 1 3-propanediol (1 3-PD) from crude glycerol. Response surface methodology (RSM) based on central composite design (CCD) was applied in designing the experiments to evaluate the interactive effects of glycerol concentration (20 to 50 g litre-1) pH (6 to 8) temperature (30C to 40C) and incubation time (48 to 72 hr) on 1 3-PD production. A total of 30 experimental runs consisting of 16 factorial points eight axial points and six centre points were conducted. It was found that the derived optimum conditions were: 39.9 g litre-1 glycerol; pH: 7.6 temperature: 33C and incubation time: 59.1 hr. Under these conditions the 1 3-PD produced (9.85 g litre-1) was 2 higher than the predicted value by RSM (9.69 g litre-1) hence the experimental design employed in validating the results obtained was significant. The analysis of variance (ANOVA) showed high coefficient of the determination values (R2) of 0.9444. The fermentation using RSM was able to increase the 1 3-PD production by two-fold.

[error in script]

Item Type:	Article
AGROVOC Term:	Biodiesel
AGROVOC Term:	Fermentation
AGROVOC Term:	Glycerol
AGROVOC Term:	Byproducts
AGROVOC Term:	Trace elements
AGROVOC Term:	Cultivation
AGROVOC Term:	Centrifugation
AGROVOC Term:	Klebsiella pneumoniae
AGROVOC Term:	Experimental design
AGROVOC Term:	Analytical methods
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 07:18
URI:	http://webagris.upm.edu.my/id/eprint/24258

Actions (login required)

View Item