Genetic variation of entomopathogenic fungi Metarhizium anisopliae and Isaria amoenerosea and their pathogenicity against subterranean termite Coptotermes curvignathus

Norman Kamarudin, . and Ramle Moslim, . and Siti Ramlah Ahmad Ali, . and Pong Kuan Kin, . and Wahizatul Afzan Azmi, . Genetic variation of entomopathogenic fungi Metarhizium anisopliae and Isaria amoenerosea and their pathogenicity against subterranean termite Coptotermes curvignathus. pp. 35-46. ISSN 1511-2780

This study determined the genetic similarity of 22 isolates of Metarhizium anisopliae and 51 isolates of Isaria amoenerosea isolated from mineral and peat soils involving random amplified polymorphic DNA (RAPD) and using polymerase chain reaction (PCR) technology. The pathogenicity of the selected isolates from both genera of fungi were then tested against the subterranean termites Coptotermes curvignathus. The RAPD-PCR analysis revealed high genetic similarity within the isolates of M. anisopliae and I. amoenerosea at the similarity ranging from 52 to 100. For M. anisopliae the fungal isolates were grouped into two main clusters and the isolates of I. amoenerosea were separated into five main clusters. Within clusters in each species there was little evidence on high similarity within isolates from the same soil type but most of fungal isolates were well distributed within the clusters. Based on the bioassay data the isolates of M. anisopliae were more pathogenic on termites as compared to the isolates of I. amoenerosea. The isolates of M. anisopliae caused high percentage of infections ranging from 71 to 84 at 15 days after treatment (DAT) and the isolate of M11H was the most pathogenic against termites with the LT50 value of 6.36 days. For isolates of I. amoenerosea the percentage of infection were low ranging from only 5 to 31. This laboratory study showed that M. anisopliae has a potential to be used to control termites as it has rapid infecting capability. However further studies should be conducted to confirm the effectiveness of the M. anisopliae to control termites especially in the field.

Full text available from: Official URL: http://jopr.mpob.gov.my/genetic-variation-of-entom...

Abstract

This study determined the genetic similarity of 22 isolates of Metarhizium anisopliae and 51 isolates of Isaria amoenerosea isolated from mineral and peat soils involving random amplified polymorphic DNA (RAPD) and using polymerase chain reaction (PCR) technology. The pathogenicity of the selected isolates from both genera of fungi were then tested against the subterranean termites Coptotermes curvignathus. The RAPD-PCR analysis revealed high genetic similarity within the isolates of M. anisopliae and I. amoenerosea at the similarity ranging from 52 to 100. For M. anisopliae the fungal isolates were grouped into two main clusters and the isolates of I. amoenerosea were separated into five main clusters. Within clusters in each species there was little evidence on high similarity within isolates from the same soil type but most of fungal isolates were well distributed within the clusters. Based on the bioassay data the isolates of M. anisopliae were more pathogenic on termites as compared to the isolates of I. amoenerosea. The isolates of M. anisopliae caused high percentage of infections ranging from 71 to 84 at 15 days after treatment (DAT) and the isolate of M11H was the most pathogenic against termites with the LT50 value of 6.36 days. For isolates of I. amoenerosea the percentage of infection were low ranging from only 5 to 31. This laboratory study showed that M. anisopliae has a potential to be used to control termites as it has rapid infecting capability. However further studies should be conducted to confirm the effectiveness of the M. anisopliae to control termites especially in the field.

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Item Type:	Article
AGROVOC Term:	Metarhizium anisopliae
AGROVOC Term:	Isaria
AGROVOC Term:	Coptotermes
AGROVOC Term:	Entomogenous fungi
AGROVOC Term:	Genetic variation
AGROVOC Term:	Biological control agents
AGROVOC Term:	Arthropods
AGROVOC Term:	Genes
AGROVOC Term:	Genetic markers
AGROVOC Term:	Insect pests
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 07:18
URI:	http://webagris.upm.edu.my/id/eprint/24262

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