Osmotic oxidative and salinity stresses upregulate the expressions of thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis)

Zainor Hafisah Che Idris, . and Wong Sook Yee, . and Zetty Norhana Balia Yusof, . and Aisamuddin Ardi Zainal Abidin, . and Nur Syuhadah Abdul Rahman, . Osmotic oxidative and salinity stresses upregulate the expressions of thiamine (vitamin B1) biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaeis guineensis). pp. 308-319. ISSN 1511-2780

Thiamine or vitamin B1 comprises a pyrimidine moiety and a thiazole moiety. Thiamine pyrophosphate (TPP) the active form of thiamine acts as a cofactor for various major enzymes for example transketolase (TK) -ketoglutarate dehydrogenase (KGDH) and pyruvate dehydrogenase (PDH). In this study the gene transcripts of first two enzymes in thiamine biosynthesis pathway THIC and THI1/THI4 were identified and amplified from oil palm tissues. Primers were designed based on sequence comparison of the genes from Arabidopsis thaliana Zea mays Oryza sativa and Alnus glutinosa. The oil palms response in terms of the expression profiles of these two thiamine biosynthesis genes to osmotic stress salinity stress and oxidative stress were analysed in this project. This is due to the suggestion that TPP plays another important role which is protecting plants against abiotic and biotic stresses. Both THIC and THI1/THI4 gene transcripts were successfully amplified and the expressions of the gene transcripts were detected using reverse transcriptase polymerase chain reaction (RT-PCR). The levels of expressions were measured and the results showed that the expression of THIC gene transcripts showed increase in gene expression in conjunction with increase concentration of stress inducer.

Full text available from: Official URL: http://jopr.mpob.gov.my/osmotic-oxidative-and-sali...

Abstract

Thiamine or vitamin B1 comprises a pyrimidine moiety and a thiazole moiety. Thiamine pyrophosphate (TPP) the active form of thiamine acts as a cofactor for various major enzymes for example transketolase (TK) -ketoglutarate dehydrogenase (KGDH) and pyruvate dehydrogenase (PDH). In this study the gene transcripts of first two enzymes in thiamine biosynthesis pathway THIC and THI1/THI4 were identified and amplified from oil palm tissues. Primers were designed based on sequence comparison of the genes from Arabidopsis thaliana Zea mays Oryza sativa and Alnus glutinosa. The oil palms response in terms of the expression profiles of these two thiamine biosynthesis genes to osmotic stress salinity stress and oxidative stress were analysed in this project. This is due to the suggestion that TPP plays another important role which is protecting plants against abiotic and biotic stresses. Both THIC and THI1/THI4 gene transcripts were successfully amplified and the expressions of the gene transcripts were detected using reverse transcriptase polymerase chain reaction (RT-PCR). The levels of expressions were measured and the results showed that the expression of THIC gene transcripts showed increase in gene expression in conjunction with increase concentration of stress inducer.

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Item Type:	Article
AGROVOC Term:	Thiamin
AGROVOC Term:	Vitamin B1
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palms
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Osmotic pressure
AGROVOC Term:	Oxidative stress
AGROVOC Term:	Genes
AGROVOC Term:	Gene expression
AGROVOC Term:	Enzymes
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 07:22
URI:	http://webagris.upm.edu.my/id/eprint/24287

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