Differences in prokaryotic species between primary and logged-over deep peat forest in Sarawak Malaysia

Nur Aziemah Ghani, . and Sakinah Safari, . and Shamsilawani Ahamed Bakeri, . and Mohamed Mazmira Mohd Masri, . and Mohd Shawal Thakib Maidin, . and Siti Ramlah Ahmad Ali, . and Sharifah Azura Syed Ibrahim, . Differences in prokaryotic species between primary and logged-over deep peat forest in Sarawak Malaysia. pp. 281-295. ISSN 1511-2780

Peatland has an important role in environmental sustainability which can be used for agricultural purposes. However deforestation in the logged-over forest may disrupt the diversity of microbial population in peat soil. Therefore this study focuses on the differences of microbial populations in Maludam primary forest and Cermat Ceria logged-over forest in Sarawak Malaysia. The prokaryotic 16S rDNA region was amplified followed by denaturing gradient gel electrophoresis (16S PCR-DGGE) analysis. Berger-Parker and Shannon-Weaver Biodiversity Index showed that Maludam (0.11 7.75) was more diverse compared to Cermat Ceria (0.19 7.63). Sequence analysis showed that the bacterial community in Maludam and Cermat Ceria were dominated by unclassified bacteria followed by Acidobacteria Actinobacteria Firmicutes and -Proteobacteria. Based on the findings the distinct species that can be found in Maludam were Acidobacterium capsulatum Solibacter sp. Mycobacterium intracellulare Rhodoplanes sp. Clostridia bacterium Exiguobacterium sp. and Lysinibacillus fusiformis. While the distinct species that can be found in Cermat Ceria were Telmatobacter Mycobacterium tuberculosis and Bacillus tequilensis. Overall the findings showed that microbial population in the logged-over forest are less diverse compared to primary forest. Higher prokaryotic diversity identified in the primary forest compared to logged-over forest showed that deforestation might cause prokaryotic population changes to both ecosystems.

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Abstract

Peatland has an important role in environmental sustainability which can be used for agricultural purposes. However deforestation in the logged-over forest may disrupt the diversity of microbial population in peat soil. Therefore this study focuses on the differences of microbial populations in Maludam primary forest and Cermat Ceria logged-over forest in Sarawak Malaysia. The prokaryotic 16S rDNA region was amplified followed by denaturing gradient gel electrophoresis (16S PCR-DGGE) analysis. Berger-Parker and Shannon-Weaver Biodiversity Index showed that Maludam (0.11 7.75) was more diverse compared to Cermat Ceria (0.19 7.63). Sequence analysis showed that the bacterial community in Maludam and Cermat Ceria were dominated by unclassified bacteria followed by Acidobacteria Actinobacteria Firmicutes and -Proteobacteria. Based on the findings the distinct species that can be found in Maludam were Acidobacterium capsulatum Solibacter sp. Mycobacterium intracellulare Rhodoplanes sp. Clostridia bacterium Exiguobacterium sp. and Lysinibacillus fusiformis. While the distinct species that can be found in Cermat Ceria were Telmatobacter Mycobacterium tuberculosis and Bacillus tequilensis. Overall the findings showed that microbial population in the logged-over forest are less diverse compared to primary forest. Higher prokaryotic diversity identified in the primary forest compared to logged-over forest showed that deforestation might cause prokaryotic population changes to both ecosystems.

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Item Type:	Article
AGROVOC Term:	Species diversity
AGROVOC Term:	Peatlands
AGROVOC Term:	Peat soils
AGROVOC Term:	DNA
AGROVOC Term:	Extraction
AGROVOC Term:	Nucleotide sequence
AGROVOC Term:	Sampling
AGROVOC Term:	Gel electrophoresis
AGROVOC Term:	Bacillus
AGROVOC Term:	Bacillaceae
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 07:22
URI:	http://webagris.upm.edu.my/id/eprint/24289

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