Citation
Loh Soh Kheang, . and Rosland Abel Stasha Eleanor, . Conversion of crude glycerol to 1 3-propanediol by newly isolated Kluyvera cryocrescens. pp. 222-227. ISSN 1511-2780
Abstract
Biodiesel an environmental-friendly and renewable fuel has gained market share and popularity as an alternative to fossil fuel. While expanding its production globally to meet the demand the production of its principal co-product crude glycerol which is surplus and under utilised has affected both the economic and environment. Crude glycerol has limited usage due to the impurities present. It cannot be disposed naturally in the environment and its storage and processing are very costly. Glycerol with its triglyceride backbone serves as a natural metabolite susceptible to microbial degradation into high value-added compounds. In this study a novel 1 3-PD producing bacterial strain isolated from palm oil mill effluent was used in microbial fermentation of crude glycerol. The strain identified as Kluyvera cryocrescens NBRC 102467 based on its 16S rRNA sequences was capable of producing 1 3-PD (5.28 g litre-1) along with by-products butanol (0.34 g litre-1) and acetone (0.31 g litre-1) after an optimum 48 hr of incubation at 30C in agitated medium enriched with crude glycerol at 150 rpm. Interestingly its productivity peaked at the 6 hr reaching 0.28 g litre-1 hr-1 and declined thereafter. In future this strain has potential to be used in the bioprocess of interest.
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Abstract
Biodiesel an environmental-friendly and renewable fuel has gained market share and popularity as an alternative to fossil fuel. While expanding its production globally to meet the demand the production of its principal co-product crude glycerol which is surplus and under utilised has affected both the economic and environment. Crude glycerol has limited usage due to the impurities present. It cannot be disposed naturally in the environment and its storage and processing are very costly. Glycerol with its triglyceride backbone serves as a natural metabolite susceptible to microbial degradation into high value-added compounds. In this study a novel 1 3-PD producing bacterial strain isolated from palm oil mill effluent was used in microbial fermentation of crude glycerol. The strain identified as Kluyvera cryocrescens NBRC 102467 based on its 16S rRNA sequences was capable of producing 1 3-PD (5.28 g litre-1) along with by-products butanol (0.34 g litre-1) and acetone (0.31 g litre-1) after an optimum 48 hr of incubation at 30C in agitated medium enriched with crude glycerol at 150 rpm. Interestingly its productivity peaked at the 6 hr reaching 0.28 g litre-1 hr-1 and declined thereafter. In future this strain has potential to be used in the bioprocess of interest.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Enterobacteriaceae |
| AGROVOC Term: | Biodiesel |
| AGROVOC Term: | Biofuels |
| AGROVOC Term: | Glycerol |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Centrifugation |
| AGROVOC Term: | Identification |
| AGROVOC Term: | Scanning microscopy |
| AGROVOC Term: | Butanol |
| AGROVOC Term: | Acetone |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/24296 |
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