Simple impeller systems for maintenance of oil palm culture aggregates

Zaiton Rasul, . and Tarmizi A. H., . and Rosli Md Yunus, . Simple impeller systems for maintenance of oil palm culture aggregates. pp. 184-190. ISSN 1511-2780

Scaling up of liquid culture systems generally involves moving from the use of simple shake flasks to bioreactors or specialised vessels; this is costly. A new innovation called the Two-in-One MPOB Simple Impeller (2-in-1 MoSLIM) was developed using commonly available Schott bottles in the laboratory. This system provided simultaneous aeration and agitation (two-inone) in a single device for tissue propagation in liquid culture. The 2-in-1 MoSLIM produced cell aggregates with fresh weight increments of two- to six-fold over 30-40 days. This system was a convenient alternative compared to the conventional shake flask system. Multiplication of cultures in the 2-in-1 MoSLIM did not require any shaker or a big space area. This system with a working volume of 300 “ 700 ml used a simple impeller and a pump for agitation and aeration purposes. However with the 2-in-1 MoSLIM media replenishment remained a tedious task. To overcome this modifications were made to the system to enable media replenishment on-site without the need of a sterile hood. The adaptation of 2-in-1 MoSLIM with an earlier innovation Fast Transfer Technique (MoFaTT) in Liquid Culture System resulted in the development of the Simple Impeller with Fast Transfer Technique (SLIM-FaTT) system. This new system can be applied to the liquid culture system of any crop with a potential towards automation.

Full text available from: Official URL: http://jopr.mpob.gov.my/simple-impeller-systems-fo...

Abstract

Scaling up of liquid culture systems generally involves moving from the use of simple shake flasks to bioreactors or specialised vessels; this is costly. A new innovation called the Two-in-One MPOB Simple Impeller (2-in-1 MoSLIM) was developed using commonly available Schott bottles in the laboratory. This system provided simultaneous aeration and agitation (two-inone) in a single device for tissue propagation in liquid culture. The 2-in-1 MoSLIM produced cell aggregates with fresh weight increments of two- to six-fold over 30-40 days. This system was a convenient alternative compared to the conventional shake flask system. Multiplication of cultures in the 2-in-1 MoSLIM did not require any shaker or a big space area. This system with a working volume of 300 “ 700 ml used a simple impeller and a pump for agitation and aeration purposes. However with the 2-in-1 MoSLIM media replenishment remained a tedious task. To overcome this modifications were made to the system to enable media replenishment on-site without the need of a sterile hood. The adaptation of 2-in-1 MoSLIM with an earlier innovation Fast Transfer Technique (MoFaTT) in Liquid Culture System resulted in the development of the Simple Impeller with Fast Transfer Technique (SLIM-FaTT) system. This new system can be applied to the liquid culture system of any crop with a potential towards automation.

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Item Type:	Article
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palms
AGROVOC Term:	Culture media
AGROVOC Term:	In vitro culture
AGROVOC Term:	In vitro regeneration
AGROVOC Term:	Aeration
AGROVOC Term:	Analysis of variance
AGROVOC Term:	Micropropagation
AGROVOC Term:	Oil plants
AGROVOC Term:	Plant growth regulators
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/24300

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