Soil fungal community associated with peat in Sarawak identified using 18S rDNA marker

Shamsilawani Ahamed Bakeri, . and Sakinah Safari, . and Nur Aziemah Ab. Ghani, . and Siti Ramlah Ahmad Ali, . and Mohd Shawal Thakib, . Soil fungal community associated with peat in Sarawak identified using 18S rDNA marker. pp. 161-171. ISSN 1511-2780

Fungi are principal decomposing microorganisms in acidic environment of peatlands. A useful tool for molecular screening of soil fungal communities using the 18S rDNA primer has been proven capable of identifying a broad range of fungi species within Ascomycota Basidiomycota Zygomycota and Chytridiomycota. Currently very little information is available on fungal communities in deep peat of Sarawak Malaysia. In this study we have isolated the fungi from soil samples taken in deep peat forests and oil palm cultivated areas. The fungal identity was undertaken using 18S rDNA primer which is EF4-F/ fung5-R. The microscopic structures were conducted to confirm the identity of the isolates. Based on this study the fungal division most commonly found in deep peat is the Ascomycota. Aspergillus fumigatus was the most common species and more dominant in oil palm cultivated areas and logged-over forest than in primary forest. In the primary forest the dominant species was the A. flavus while Hypocrea atroviridis was commonly associated with oil palm cultivated areas and logged-over forest. Other species of fungi isolated in peat primary forests were Penicillium chrysogenum Trichoderma sp. Phanerochaete sp. Mortierella chlamydospora A. niger A. alliaceus etc. The in-depth difference in the fungal communities for the different sites will be further investigated using the next generation sequencing technology.

Full text available from: Official URL: http://jopr.mpob.gov.my/soil-fungal-community-asso...

Abstract

Fungi are principal decomposing microorganisms in acidic environment of peatlands. A useful tool for molecular screening of soil fungal communities using the 18S rDNA primer has been proven capable of identifying a broad range of fungi species within Ascomycota Basidiomycota Zygomycota and Chytridiomycota. Currently very little information is available on fungal communities in deep peat of Sarawak Malaysia. In this study we have isolated the fungi from soil samples taken in deep peat forests and oil palm cultivated areas. The fungal identity was undertaken using 18S rDNA primer which is EF4-F/ fung5-R. The microscopic structures were conducted to confirm the identity of the isolates. Based on this study the fungal division most commonly found in deep peat is the Ascomycota. Aspergillus fumigatus was the most common species and more dominant in oil palm cultivated areas and logged-over forest than in primary forest. In the primary forest the dominant species was the A. flavus while Hypocrea atroviridis was commonly associated with oil palm cultivated areas and logged-over forest. Other species of fungi isolated in peat primary forests were Penicillium chrysogenum Trichoderma sp. Phanerochaete sp. Mortierella chlamydospora A. niger A. alliaceus etc. The in-depth difference in the fungal communities for the different sites will be further investigated using the next generation sequencing technology.

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Item Type:	Article
AGROVOC Term:	Fungi
AGROVOC Term:	Peat
AGROVOC Term:	Decomposition
AGROVOC Term:	Peat soils
AGROVOC Term:	Identification
AGROVOC Term:	Isolation
AGROVOC Term:	DNA
AGROVOC Term:	Extraction
AGROVOC Term:	Purification
AGROVOC Term:	Sampling
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/24303

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