Oil palm (Elaeis guineensis Jacq.) improvement: pollen assessment for better conservation and germination

Frank George, . and Tabi Kingsley, . and Tonfack Libert Brice, . and Youmbi Emmanuel, . and Ebongue Ngando, . and Ntsomboh Godswill, . Oil palm (Elaeis guineensis Jacq.) improvement: pollen assessment for better conservation and germination. pp. 212-219. ISSN 1511-2780

Planting of improved Elaeis guineensis seeds to increase oil production is a sustainable strategy to mitigate the impact of crop-based demand for bioenergy. But breeders need a quality pollen stock for successful hybridisation of outcrossing plants. This study was carried out to determine suitable conditions for in vitro germination and conservation of oil palm pollen. Performance of pollen germination was measured in three distinct culture media: Brewbaker and Kwack (BK) Heslop-Harrison (HH) and Arnaud (FA) media each supplemented with seven sucrose concentration suc (0 0.05 0.1 0.15 0.2 0.25 30) for five incubation durations (1-5 hr). Optimal germination scores of 73 41 and 39 for BK (15 suc) HH and FA (10 suc) were obtained respectively after 3 hr of incubation at 38C. Fresh pollen was also treated to four moisture content (MC) ranges: 100 78-70 65-57 and 41-3 on fresh weight (FW) basis. In vitro germination scores of 81 0.83 56 and 29 were obtained respectively when cultured in BK (15 suc) after 3 hr of incubation. After 50 days of storage pollen with MC 78-70 scored in vitro germination of 70 and 3 at -20C and 10C respectively. These results precisely determine optimal conditions for oil palm pollen ex situ conservation thereby optimising its usage.

Full text available from: Official URL: http://jopr.mpob.gov.my/oil-palm-elaeis-guineensis...

Abstract

Planting of improved Elaeis guineensis seeds to increase oil production is a sustainable strategy to mitigate the impact of crop-based demand for bioenergy. But breeders need a quality pollen stock for successful hybridisation of outcrossing plants. This study was carried out to determine suitable conditions for in vitro germination and conservation of oil palm pollen. Performance of pollen germination was measured in three distinct culture media: Brewbaker and Kwack (BK) Heslop-Harrison (HH) and Arnaud (FA) media each supplemented with seven sucrose concentration suc (0 0.05 0.1 0.15 0.2 0.25 30) for five incubation durations (1-5 hr). Optimal germination scores of 73 41 and 39 for BK (15 suc) HH and FA (10 suc) were obtained respectively after 3 hr of incubation at 38C. Fresh pollen was also treated to four moisture content (MC) ranges: 100 78-70 65-57 and 41-3 on fresh weight (FW) basis. In vitro germination scores of 81 0.83 56 and 29 were obtained respectively when cultured in BK (15 suc) after 3 hr of incubation. After 50 days of storage pollen with MC 78-70 scored in vitro germination of 70 and 3 at -20C and 10C respectively. These results precisely determine optimal conditions for oil palm pollen ex situ conservation thereby optimising its usage.

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Item Type:	Article
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palms
AGROVOC Term:	Pollen culture
AGROVOC Term:	Varieties
AGROVOC Term:	Culture media
AGROVOC Term:	Germination
AGROVOC Term:	In vitro culture
AGROVOC Term:	In vitro regeneration
AGROVOC Term:	Experimental design
AGROVOC Term:	Germination rate
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/24342

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