Characterisation of 13 polymorphic simple sequence repeat (SSR) markers for cassava (Manihot esculenta Crantz)

Muhammad Fairuz Mohd Yusof, . and Noor Syahira Nasarudin, . and Mohd Azwan Jaafar, . and Siti Norhayati Ismail, . and Mohd Shahril Firdaus Ab Razak, . Characterisation of 13 polymorphic simple sequence repeat (SSR) markers for cassava (Manihot esculenta Crantz). pp. 17-23. ISSN 1394-9829

Cassava is widely cultivated as an annual crop for carbohydrates source in tropical and subtropical regions. It provides food security against famine due to its inherent tolerance to stressful environments. The present study aims to provide preliminary information on 13 polymorphic SSR markers to both researchers and breeders as it is essentially useful to manage their breeding program. These 13 SSR markers displayed a wide range of polymorphism whereby the number of alleles ranged from 2 (SSRY106) to 10 (SSRY324) with an average of 5.5 alleles per locus. The highest polymorphism information content (PIC) is shown in marker SSRY324 with the value of 0.9999 while SSRY106 showed the lowest value of 0.3893. The observed and expected heterozygosity varied from 0.2549 to 0.9391 and 0.3902 to 0.8192 with an average of 0.6309 and 0.6067 respectively. These markers indicated sufficient levels of polymorphism that could further be employed in studies on population and genetic variation as well as cultivar identification of cassava in Malaysia.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/46-1/cassava.pdf

Abstract

Cassava is widely cultivated as an annual crop for carbohydrates source in tropical and subtropical regions. It provides food security against famine due to its inherent tolerance to stressful environments. The present study aims to provide preliminary information on 13 polymorphic SSR markers to both researchers and breeders as it is essentially useful to manage their breeding program. These 13 SSR markers displayed a wide range of polymorphism whereby the number of alleles ranged from 2 (SSRY106) to 10 (SSRY324) with an average of 5.5 alleles per locus. The highest polymorphism information content (PIC) is shown in marker SSRY324 with the value of 0.9999 while SSRY106 showed the lowest value of 0.3893. The observed and expected heterozygosity varied from 0.2549 to 0.9391 and 0.3902 to 0.8192 with an average of 0.6309 and 0.6067 respectively. These markers indicated sufficient levels of polymorphism that could further be employed in studies on population and genetic variation as well as cultivar identification of cassava in Malaysia.

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Item Type:	Article
AGROVOC Term:	Cassava
AGROVOC Term:	Genetic markers
AGROVOC Term:	Plant breeding
AGROVOC Term:	Plant breeders
AGROVOC Term:	Polymorphism
AGROVOC Term:	Alleles
AGROVOC Term:	Genetic variation
AGROVOC Term:	Cultivars
AGROVOC Term:	Genetic diversity within species
AGROVOC Term:	Manihot esculenta
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	30 Apr 2025 01:15
URI:	http://webagris.upm.edu.my/id/eprint/24362

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