Citation
Hasnisa Hashim, . and Arif Zaidi Jusoh, . and Ahmad Tarmizi Salimin, . and Mohd Nazrul Hisham Daud, . and Suri Roowi, . and Hadijah Hassan, . and Syahida Maarof, . and Nurul Nabilah Mohd Fiteri, . and Sharizan Ahmad, . and Kharis Zahid, . In-vivo toxicity studies of a mixture of Hibiscus sabdariffa L. Clinacanthus nutans L. and Stevia leaves in Sprague Dawley rats. pp. 111-119. ISSN 1394-9829
Abstract
Natural products from medicinal plants are widely used worldwide as they are claimed to generate new alternative medicines. Following its proven medicinal properties a mixture of Hibiscus sabdariffa L. Clinacanthus nutans L. Stevia rebaudiana in the ratio of 1:1:0.5 (HS:CN:Stevia; w/w/w) was shown to be effective therapy as diuretic and in chemoprevention. However no study has been conducted to assess the toxic effect or the LD50 of this mixture. Therefore a standard in-vivo toxicological assessment was carried out in a 28-day oral administration study. The doses tested were 1 000 mg/kg 2 000 mg/kg and 5 000 mg/kg of body weight in Sprague Dawley rats. No mortality adverse clinical signs and abnormal changes in body weight. Toxicologically significant changes were only seen in relative liver weight red blood cell count haematocrit haemoglobin and total protein. However the difference was toxicologically insignificant since the values were within normal physiological ranges. These observations suggest that the mixture is practically nontoxic in Sprague Dawley rats and the no-observed-adverse-effect level (NOAEL) is greater than 5 000 mg/kg.
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Abstract
Natural products from medicinal plants are widely used worldwide as they are claimed to generate new alternative medicines. Following its proven medicinal properties a mixture of Hibiscus sabdariffa L. Clinacanthus nutans L. Stevia rebaudiana in the ratio of 1:1:0.5 (HS:CN:Stevia; w/w/w) was shown to be effective therapy as diuretic and in chemoprevention. However no study has been conducted to assess the toxic effect or the LD50 of this mixture. Therefore a standard in-vivo toxicological assessment was carried out in a 28-day oral administration study. The doses tested were 1 000 mg/kg 2 000 mg/kg and 5 000 mg/kg of body weight in Sprague Dawley rats. No mortality adverse clinical signs and abnormal changes in body weight. Toxicologically significant changes were only seen in relative liver weight red blood cell count haematocrit haemoglobin and total protein. However the difference was toxicologically insignificant since the values were within normal physiological ranges. These observations suggest that the mixture is practically nontoxic in Sprague Dawley rats and the no-observed-adverse-effect level (NOAEL) is greater than 5 000 mg/kg.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hibiscus sabdariffa |
| AGROVOC Term: | Stevia |
| AGROVOC Term: | Toxicity |
| AGROVOC Term: | In vivo experimentation |
| AGROVOC Term: | Rats |
| AGROVOC Term: | Medicinal plants |
| AGROVOC Term: | Medicinal properties |
| AGROVOC Term: | Diuretics |
| AGROVOC Term: | Body weight |
| AGROVOC Term: | Dosage |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 30 Apr 2025 01:19 |
| URI: | http://webagris.upm.edu.my/id/eprint/24375 |
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