On-line high-performance liquid chromatography analysis of the antioxidant activity of phenolic compounds in selected tropical citrus

Suri Roowi, . and Crozier Alan, . and Zaharinah Hussin, . and Zuraida Abd. Rahman, . On-line high-performance liquid chromatography analysis of the antioxidant activity of phenolic compounds in selected tropical citrus. pp. 81-94. ISSN 1394-9829

Citrus hystrix Citrus microcarpa and Citrus suhuiensis fruits were analysed by high-performance liquid chromatography (HPLC) with photo-diode array and MS2 detector (HPLC-PDA-MS2) to identify flavone flavanone and dihydrochalcone. The antioxidant potential on each tropical citrus flavonoid was performed using HPLC with post-column on-line antioxidant detection based on 2 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay. Phloretin-3 5-di-C-glucopyranoside present in C. microcarpa fruit peel and flesh possess high Trolox Equivalent Antioxidant Ratio (TEAR) values by 3.4 and 3.1 respectively. Quercetin-3-O-rutinoside hesperetin-7-Oneohesperidoside and hesperetin-7-O-rutinoside also showed high TEAR values in C. hystrix and C. suhuiensis fruit but have lower than phloretin-3 5-diC-glucopyranoside. Levels of TEAR values of flavonoids in C. microcarpa C. suhuiensis and C. hystrix fruits were as follows; phloretin-3 5-di-Cglucopyranoside quercetin-3-O-rutinoside hesperetin-7-O-neohesperidoside hesperetin-7-O-rutinoside luteolin-7-O-rutinoside naringenin-7-Orutinoside. Citrus microcarpa has been widely used in Asian food and beverages or as a preserved snack is consider to be a good source of phloretin-33 5-di-Cglucopyranoside. This compound is structurally almost similar to aspalathin and nothofagin the potent antioxidant compounds in rooibos tea.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/44-1/online.pdf

Abstract

Citrus hystrix Citrus microcarpa and Citrus suhuiensis fruits were analysed by high-performance liquid chromatography (HPLC) with photo-diode array and MS2 detector (HPLC-PDA-MS2) to identify flavone flavanone and dihydrochalcone. The antioxidant potential on each tropical citrus flavonoid was performed using HPLC with post-column on-line antioxidant detection based on 2 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay. Phloretin-3 5-di-C-glucopyranoside present in C. microcarpa fruit peel and flesh possess high Trolox Equivalent Antioxidant Ratio (TEAR) values by 3.4 and 3.1 respectively. Quercetin-3-O-rutinoside hesperetin-7-Oneohesperidoside and hesperetin-7-O-rutinoside also showed high TEAR values in C. hystrix and C. suhuiensis fruit but have lower than phloretin-3 5-diC-glucopyranoside. Levels of TEAR values of flavonoids in C. microcarpa C. suhuiensis and C. hystrix fruits were as follows; phloretin-3 5-di-Cglucopyranoside quercetin-3-O-rutinoside hesperetin-7-O-neohesperidoside hesperetin-7-O-rutinoside luteolin-7-O-rutinoside naringenin-7-Orutinoside. Citrus microcarpa has been widely used in Asian food and beverages or as a preserved snack is consider to be a good source of phloretin-33 5-di-Cglucopyranoside. This compound is structurally almost similar to aspalathin and nothofagin the potent antioxidant compounds in rooibos tea.

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Item Type:	Article
AGROVOC Term:	Citrus
AGROVOC Term:	Antioxidants
AGROVOC Term:	Phenolic compounds
AGROVOC Term:	High performance liquid chromatography
AGROVOC Term:	Citrus microcarpa
AGROVOC Term:	Flavones
AGROVOC Term:	Dihydrochalcones
AGROVOC Term:	Free radicals
AGROVOC Term:	Chemical structure
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24407

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