Identification of candidate genes involved in brown planthopper resistance in rice using microarray analysis

Mohamad Bahagia Ab Ghaffar, . and Norliza Tendot Abu Bakar, . and Pritchard J., . and Ford Lloyd B., . Identification of candidate genes involved in brown planthopper resistance in rice using microarray analysis. pp. 49-62. ISSN 1394-9829

Recent development in rice genomics has greatly facilitated new technologies to be used to decipher the complex resistance mechanisms to rice brown planthopper (BPH) Nilaparvata lugens (Stal). In this study microarray analysis using a 44K Agilent chip was conducted on 12 rice varieties having different levels of BPH resistance. Results revealed the presence of differences in expressed genes between resistant and susceptible varieties which coincide very well with the previous physiological results obtained via Electrical Penetration Graph (EPG) and honeydew clock experiments. A total of 21 556 probes passed a statistical filter using the quantile method. A total of 239 probes were identified as being significantly different in expression between the resistant and susceptible varieties. The expression of 89 genes was up-regulated while 150 genes were down regulated. Some of the genes identified were highly correlated with the phenotypic data obtained in earlier studies. Among them are hexose transporter (stp1) (R 0.71) and glutamate receptor protein GLR3.4b (GLR3.4) (R “ 0.69) genes which are highly correlated with the phloem ingestion EPG waveform type. The microarray analysis on the 12 rice varieties and the potential candidate genes that may play their roles in BPH resistance for future study were discussed.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/44-1/identificatio...

Abstract

Recent development in rice genomics has greatly facilitated new technologies to be used to decipher the complex resistance mechanisms to rice brown planthopper (BPH) Nilaparvata lugens (Stal). In this study microarray analysis using a 44K Agilent chip was conducted on 12 rice varieties having different levels of BPH resistance. Results revealed the presence of differences in expressed genes between resistant and susceptible varieties which coincide very well with the previous physiological results obtained via Electrical Penetration Graph (EPG) and honeydew clock experiments. A total of 21 556 probes passed a statistical filter using the quantile method. A total of 239 probes were identified as being significantly different in expression between the resistant and susceptible varieties. The expression of 89 genes was up-regulated while 150 genes were down regulated. Some of the genes identified were highly correlated with the phenotypic data obtained in earlier studies. Among them are hexose transporter (stp1) (R 0.71) and glutamate receptor protein GLR3.4b (GLR3.4) (R “ 0.69) genes which are highly correlated with the phloem ingestion EPG waveform type. The microarray analysis on the 12 rice varieties and the potential candidate genes that may play their roles in BPH resistance for future study were discussed.

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Item Type:	Article
AGROVOC Term:	Plant hoppers
AGROVOC Term:	Rice
AGROVOC Term:	Genes
AGROVOC Term:	Identification
AGROVOC Term:	genomics
AGROVOC Term:	Nilaparvata lugens
AGROVOC Term:	Genes
AGROVOC Term:	Phenotypes
AGROVOC Term:	Glutamates
AGROVOC Term:	Phloem
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24409

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