Lysis activity of bacteriophages isolated from raw meat and their potential as biocontrol agents against Escherichia coli

Nur Baizura Sa’dom, . and Asiah Abu Samah, . and Mohd Effendi Mohamed Noor, . and Nur Ilida Mohamad, . and Tan G. H., . Lysis activity of bacteriophages isolated from raw meat and their potential as biocontrol agents against Escherichia coli. pp. 55-60. ISSN 1394-9829

The potential application of bacteriophages as biological agent in controlling pathogenic bacteria for improving food safety appears to gain interest and recognition quite recently. This study was to evaluate the lysis activity of bacteriophages isolated from raw meat. The lysis activity evaluated using disc diffusion method and in vitro challenge test in LB broth showed that the isolated bacteriophages have the ability to control the growth of Escherichia coli ATCC 25922 thus have potential as biological control agent for future use in food industry. The isolated bacteriophages have the ability to lyse E. coli ATCC 25922 cells culture in LB broth at 37 C and 30 C during 4 h of incubation. This finding is supported by the turbidity result which also indicates that the bacteriophages suppressed the growth of E. coli cells in LB broth. On the other hand no lysis activity could be observed at 4 C. However more precise studies need to be done on all isolates to confirm on their potential as biological control of E. coli in food industry in the near future.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/43-1/lysis.pdf

Abstract

The potential application of bacteriophages as biological agent in controlling pathogenic bacteria for improving food safety appears to gain interest and recognition quite recently. This study was to evaluate the lysis activity of bacteriophages isolated from raw meat. The lysis activity evaluated using disc diffusion method and in vitro challenge test in LB broth showed that the isolated bacteriophages have the ability to control the growth of Escherichia coli ATCC 25922 thus have potential as biological control agent for future use in food industry. The isolated bacteriophages have the ability to lyse E. coli ATCC 25922 cells culture in LB broth at 37 C and 30 C during 4 h of incubation. This finding is supported by the turbidity result which also indicates that the bacteriophages suppressed the growth of E. coli cells in LB broth. On the other hand no lysis activity could be observed at 4 C. However more precise studies need to be done on all isolates to confirm on their potential as biological control of E. coli in food industry in the near future.

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Item Type:	Article
AGROVOC Term:	Escherichia coli
AGROVOC Term:	Biocontrol
AGROVOC Term:	Bacteriophages
AGROVOC Term:	Fresh meat
AGROVOC Term:	Isolation
AGROVOC Term:	Pathogenic bacteria
AGROVOC Term:	Food safety
AGROVOC Term:	Diffusion
AGROVOC Term:	In vitro
AGROVOC Term:	Growth control
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24417

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