Statistical optimisation of radial growth rates of Ganoderma neo-japonicum (KLUM61076) in low cost solid agar plates medium using full factorial design and central composite design

Noorlidah Abdullah, . and Nik Hafizah Nik Ubaidillah, . and Muhammad Suffian Muhammad Annuar, . and Vikineswary Sabaratnam, . Statistical optimisation of radial growth rates of Ganoderma neo-japonicum (KLUM61076) in low cost solid agar plates medium using full factorial design and central composite design. pp. 61-72. ISSN 1394-9829

The combination of low cost medium sources (brown sugar spent yeast molasses and corn steep liquor) for growth of Ganoderma neo-japonicum (KLUM61076) in solid agar plates was studied. The mathematical model of Full Factorial Design and Response Surface Methodology were used to select the optimum combination of carbon/nitrogen sources for the cultivation of G. neo-japonicum. The combination medium of brown sugar and spent yeast was the best for growth of G. neo-japonicum at 5.74 (w/v) carbon content in brown sugar and 0.06 carbon content (w/v) in spent yeast. The combination of two constituents contributed to a low requirement of C/N ratio 1.74 and gave the fastest mycelial growth rates (20.74 mm/day). This combinations of spent yeast/brown sugars showed an increment in mycelia yield which is up to two-fold as compared with the standard commercial medium (yeast extract-glucose). The low cost medium has the potential to contribute to cost-effective cultivation for G. neo-japonicum large-scale production.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/43-1/ganoderma.pdf

Abstract

The combination of low cost medium sources (brown sugar spent yeast molasses and corn steep liquor) for growth of Ganoderma neo-japonicum (KLUM61076) in solid agar plates was studied. The mathematical model of Full Factorial Design and Response Surface Methodology were used to select the optimum combination of carbon/nitrogen sources for the cultivation of G. neo-japonicum. The combination medium of brown sugar and spent yeast was the best for growth of G. neo-japonicum at 5.74 (w/v) carbon content in brown sugar and 0.06 carbon content (w/v) in spent yeast. The combination of two constituents contributed to a low requirement of C/N ratio 1.74 and gave the fastest mycelial growth rates (20.74 mm/day). This combinations of spent yeast/brown sugars showed an increment in mycelia yield which is up to two-fold as compared with the standard commercial medium (yeast extract-glucose). The low cost medium has the potential to contribute to cost-effective cultivation for G. neo-japonicum large-scale production.

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Item Type:	Article
AGROVOC Term:	Ganoderma
AGROVOC Term:	Growth rate
AGROVOC Term:	Agar
AGROVOC Term:	Factorial analysis
AGROVOC Term:	Yeasts
AGROVOC Term:	Molasses
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24419

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