Field and laboratory detection of clove Sumatra disease caused by Ralstonia syzygii subsp. Syzygii in Java, Indonesia

Tri Joko, . and Arzaq Prabantoro Yuantomoputro, . and Restu Indrawati, . and Alan Soffan, . and Siti Subandiyah, . (2023) Field and laboratory detection of clove Sumatra disease caused by Ralstonia syzygii subsp. Syzygii in Java, Indonesia. Pertanika Journal Tropical Agricultural Science (Malaysia), 46 (3). pp. 799-813. ISSN 1511-3701

The significant decrease in clove production in Indonesia is mainly due to the Sumatra disease caused by bacterial Ralstonia syzygii subsp. syzygii. It is necessary to manage the disease broadly based on disease detection in the field and the laboratory. This study aims to determine the technique for detecting the distribution pattern of Sumatra disease using geographic information systems and validate the presence of R. syzygii subsp. syzygii in clove plant tissues by molecular analyses based on the endoglucanase gene. This research was conducted by acquiring aerial photos using uncrewed aerial vehicles processed using photogrammetric techniques to produce geographic information system outputs as a thematic map of the clove Sumatra disease distribution pattern. The plant samples were collected for molecular analysis of the pathogens causing clove Sumatra disease in the laboratory. DNA extraction was performed and amplified by polymerase chain reaction (PCR) using UGMRss-specific primers followed by Sanger sequencing. The aerial photo images showed that the distribution of clove Sumatra disease has a random pattern, which tends to spread between healthy and diseased plants. Furthermore, the PCR and phylogenetic analyses revealed that all symptomatic clove plant samples (leaves, twigs, and roots) were positively infected by R. syzygii subsp. Syzygii.

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Abstract

The significant decrease in clove production in Indonesia is mainly due to the Sumatra disease caused by bacterial Ralstonia syzygii subsp. syzygii. It is necessary to manage the disease broadly based on disease detection in the field and the laboratory. This study aims to determine the technique for detecting the distribution pattern of Sumatra disease using geographic information systems and validate the presence of R. syzygii subsp. syzygii in clove plant tissues by molecular analyses based on the endoglucanase gene. This research was conducted by acquiring aerial photos using uncrewed aerial vehicles processed using photogrammetric techniques to produce geographic information system outputs as a thematic map of the clove Sumatra disease distribution pattern. The plant samples were collected for molecular analysis of the pathogens causing clove Sumatra disease in the laboratory. DNA extraction was performed and amplified by polymerase chain reaction (PCR) using UGMRss-specific primers followed by Sanger sequencing. The aerial photo images showed that the distribution of clove Sumatra disease has a random pattern, which tends to spread between healthy and diseased plants. Furthermore, the PCR and phylogenetic analyses revealed that all symptomatic clove plant samples (leaves, twigs, and roots) were positively infected by R. syzygii subsp. Syzygii.

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Item Type:	Article
AGROVOC Term:	cloves
AGROVOC Term:	Java
AGROVOC Term:	field experimentation
AGROVOC Term:	laboratory experimentation
AGROVOC Term:	pathogens
AGROVOC Term:	geographical distribution
Geographical Term:	Indonesia
Uncontrolled Keywords:	Sumatra disease, Ralstonia syzygii
Depositing User:	Ms. Azariah Hashim
Date Deposited:	12 Feb 2025 03:20
Last Modified:	12 Feb 2025 03:20
URI:	http://webagris.upm.edu.my/id/eprint/2442

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