Expression of defense-related genes in papaya seedling infected with Erwinia mallotivora using real-time PCR

Wee Chien Yeong, . and Muhammad Hanam Hamid, . and Mohd Waznul Adly Mohd Zaidan, . and Khairun Hisam Nasir, . Expression of defense-related genes in papaya seedling infected with Erwinia mallotivora using real-time PCR. pp. 73-82. ISSN 1394-9829

Papaya is an important fruit crop in Malaysia for the domestic and export markets. The papaya industry is currently facing a major threat from the papaya dieback disease for which no effective control measure is known to prevent further spreading of the disease. The causal agent of the disease Erwinia mallotivora was only identified lately. Hence to understand the molecular mechanism underlying the host-pathogen interaction leaves of papaya seedling were infected with a suspension of E. mallotivora at the concentration of 106 cfu and subsequently the expression profiles of four identified potential defenserelated genes namely peroxidase aquaporin leucine-rich protein and zinc finger protein were analysed using quantitative real-time PCR. The expression profiles were captured at seven different time points post-infection at 0 4 8 12 24 48 and 72 h respectively. Among the four defense-related genes analysed peroxidase zinc finger protein and leucine-rich protein genes were found to be up-regulated at early stage of infection when compared to control. Meanwhile the expression of the aquaporin gene was found to be significantly up-regulated at later stage in response to E. mallotivora infection.

Full text available from: Official URL: http://ejtafs.mardi.gov.my/jtafs/42-1/Papaya%20see...

Abstract

Papaya is an important fruit crop in Malaysia for the domestic and export markets. The papaya industry is currently facing a major threat from the papaya dieback disease for which no effective control measure is known to prevent further spreading of the disease. The causal agent of the disease Erwinia mallotivora was only identified lately. Hence to understand the molecular mechanism underlying the host-pathogen interaction leaves of papaya seedling were infected with a suspension of E. mallotivora at the concentration of 106 cfu and subsequently the expression profiles of four identified potential defenserelated genes namely peroxidase aquaporin leucine-rich protein and zinc finger protein were analysed using quantitative real-time PCR. The expression profiles were captured at seven different time points post-infection at 0 4 8 12 24 48 and 72 h respectively. Among the four defense-related genes analysed peroxidase zinc finger protein and leucine-rich protein genes were found to be up-regulated at early stage of infection when compared to control. Meanwhile the expression of the aquaporin gene was found to be significantly up-regulated at later stage in response to E. mallotivora infection.

[error in script]

Item Type:	Article
AGROVOC Term:	Erwinia
AGROVOC Term:	Papayas
AGROVOC Term:	Seedlings
AGROVOC Term:	Gene expression
AGROVOC Term:	PCR
AGROVOC Term:	Dieback
AGROVOC Term:	Plant diseases
AGROVOC Term:	Pathogens
AGROVOC Term:	Infection
AGROVOC Term:	Peroxidases
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24435

Actions (login required)

View Item