Citation
Aminuddin Hussin, . and Mohd Khan Ayob, . and Madzlan Kasran, . Effects of pH on mycelial growth and amino acid composition of Lentinula edodes in submerged cultures. pp. 63-70. ISSN 1394-9829
Abstract
Lentinula edodes (Shiitake) and its mycelia have long been valued in Asian cultures due to its therapeutic as well as culinary properties. The effect of different pH values medium formulations on yield of dry mycelia and total amino acid content were studied. Submerged culture technique was employed to grow mycelia in Potato Dextrose Broth PDB and PDB (PDB yeast extract malt extract peptone KH2PO4 and MgSO4.7H2O) media under different pH values. The amino acid composition of the mycelia was determined by High Performance Liquid Chromatography (HPLC) techniques. An optimum growth of the mycelia was observed on the PDB medium incubated at pH 6. The mycelia harvested had the highest content of amino acid in which the major component was glutamic acid.
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Abstract
Lentinula edodes (Shiitake) and its mycelia have long been valued in Asian cultures due to its therapeutic as well as culinary properties. The effect of different pH values medium formulations on yield of dry mycelia and total amino acid content were studied. Submerged culture technique was employed to grow mycelia in Potato Dextrose Broth PDB and PDB (PDB yeast extract malt extract peptone KH2PO4 and MgSO4.7H2O) media under different pH values. The amino acid composition of the mycelia was determined by High Performance Liquid Chromatography (HPLC) techniques. An optimum growth of the mycelia was observed on the PDB medium incubated at pH 6. The mycelia harvested had the highest content of amino acid in which the major component was glutamic acid.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Lentinula edodes |
| AGROVOC Term: | Amino acids |
| AGROVOC Term: | Shiitake |
| AGROVOC Term: | Therapeutic substances |
| AGROVOC Term: | Flavouring crops (culinary) |
| AGROVOC Term: | HPLC |
| AGROVOC Term: | High performance liquid chromatography |
| AGROVOC Term: | Glutamic acid |
| AGROVOC Term: | Mushrooms |
| AGROVOC Term: | Glutamic acid |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24461 |
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