Citation
Nurulhuda Md Ozman, . and Roziatul Erin Abdul Razak, . and Mohd Hifzan Rosali, . and Wan Somarny Wan Md Zain, . and Ainu Husna M. S. Suhaimi, . A study of major prolificacy genes in Malin and Dorper sheep in Malaysia. pp. 265-272. ISSN 1394-9829
Abstract
High prolificacy in some sheep breeds was due to mutation in FecB and FecX genes. The mutations of these genes were associated with an increase in ovulation rate litter size and the number of follicles in the ovaries. The FecB gene was present in some high prolific breeds but absent in low prolific sheep breeds. The objective of this study was to verify the presence of these prolificacy genes and identify if the genes are polymorphic in Malin and Dorper sheep. Five prolificacy genes FecB FecXI FecXH FecXB and FecXG were screened in Malin and Dorper sheep. Four genes were successfully amplified by PCR except for FecXG which failed to amplify in all samples tested. Analysis of PCR products by Forced Restriction Fragment Length Polymorphism PCR (PCR RFLP) showed the absence of FecB and FecX gene mutations after the samples were digested with enzymes. From the analysis carried out it was shown that Malin and Dorper sheep were homozygous non-carriers for Booroola FecB and FecX gene mutations. Based on these findings both Malin and Dorper sheep breeds can be considered as low prolificacy sheep breeds.
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Abstract
High prolificacy in some sheep breeds was due to mutation in FecB and FecX genes. The mutations of these genes were associated with an increase in ovulation rate litter size and the number of follicles in the ovaries. The FecB gene was present in some high prolific breeds but absent in low prolific sheep breeds. The objective of this study was to verify the presence of these prolificacy genes and identify if the genes are polymorphic in Malin and Dorper sheep. Five prolificacy genes FecB FecXI FecXH FecXB and FecXG were screened in Malin and Dorper sheep. Four genes were successfully amplified by PCR except for FecXG which failed to amplify in all samples tested. Analysis of PCR products by Forced Restriction Fragment Length Polymorphism PCR (PCR RFLP) showed the absence of FecB and FecX gene mutations after the samples were digested with enzymes. From the analysis carried out it was shown that Malin and Dorper sheep were homozygous non-carriers for Booroola FecB and FecX gene mutations. Based on these findings both Malin and Dorper sheep breeds can be considered as low prolificacy sheep breeds.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Sheep |
| AGROVOC Term: | Ovulation |
| AGROVOC Term: | Litter size |
| AGROVOC Term: | Mutation |
| AGROVOC Term: | Breeds |
| AGROVOC Term: | Genes |
| AGROVOC Term: | Ovarian follicles |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Polymorphism |
| AGROVOC Term: | Molecular weight |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24475 |
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