as1-Casein gene polymorphism in Katjang Jamnapari Boer and Boer-feral goats in Malaysia

Amie Marini Abu Bakar, . and Abdul Rashid Baba, . and Musaddin Kamaruddin, . and Zawawi Ismail, . as1-Casein gene polymorphism in Katjang Jamnapari Boer and Boer-feral goats in Malaysia. pp. 1-5. ISSN 1394-9829

Casein and milk serum or whey proteins are the two major proteins (about 95) in mammalian milk. The casein protein comprises as1 as2 b and k-casein while b-lactoglobulin and a-lactalbumin make up the whey protein. A study was carried out to characterise and determine the as1-casein protein gene polymorphism and genetic relationship in goat breeds available in Malaysia. A total of 115 heads of goats including Katjang Boer Boer Feral and Jamnapari were used in the study. Genomic DNA was extracted from the blood samples and amplified by PCR. Genetic variability was calculated using Popgene Software computer program. This study showed that casein gene exists in goat breeds found in Malaysia. All local goat breeds showed high frequency of as1-casein A B and C alleles which are related to high casein content in milk. The highest frequency of as1-casein A alleles was found in Katjang goats with a value of 0.61. The dendogram constructed based on genetic distance matrix using neighbour-joining method showed the genetic relation between the four breeds of goats. Boer and BoerFeral goats were grouped together in one cluster whereas Katjang and Jamnapari populations were grouped in another cluster. The Boer goats differed genetically from the local Katjang or Jamnapari goats in as1-casein gene.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/39-1/Gene%20Polymo...

Abstract

Casein and milk serum or whey proteins are the two major proteins (about 95) in mammalian milk. The casein protein comprises as1 as2 b and k-casein while b-lactoglobulin and a-lactalbumin make up the whey protein. A study was carried out to characterise and determine the as1-casein protein gene polymorphism and genetic relationship in goat breeds available in Malaysia. A total of 115 heads of goats including Katjang Boer Boer Feral and Jamnapari were used in the study. Genomic DNA was extracted from the blood samples and amplified by PCR. Genetic variability was calculated using Popgene Software computer program. This study showed that casein gene exists in goat breeds found in Malaysia. All local goat breeds showed high frequency of as1-casein A B and C alleles which are related to high casein content in milk. The highest frequency of as1-casein A alleles was found in Katjang goats with a value of 0.61. The dendogram constructed based on genetic distance matrix using neighbour-joining method showed the genetic relation between the four breeds of goats. Boer and BoerFeral goats were grouped together in one cluster whereas Katjang and Jamnapari populations were grouped in another cluster. The Boer goats differed genetically from the local Katjang or Jamnapari goats in as1-casein gene.

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Item Type:	Article
AGROVOC Term:	Polymorphism
AGROVOC Term:	Goats
AGROVOC Term:	Casein
AGROVOC Term:	Whey protein
AGROVOC Term:	Beta lactoglobulin
AGROVOC Term:	Lactalbumin
AGROVOC Term:	Polymorphism
AGROVOC Term:	Breeds
AGROVOC Term:	genomics
AGROVOC Term:	DNA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24513

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