Identification and isolation of methyl gallate as a polar chemical marker for Labisia pumila Benth.


Citation

Normah Ahamad, . and Mohd Lip Jabit, . and Nurul Nabilah Mohd Fiteri, . and Mohd Nazrul Hisham Daud, . and Mohd Noh Jalil, . Identification and isolation of methyl gallate as a polar chemical marker for Labisia pumila Benth. pp. 279-284. ISSN 1394-9829

Abstract

This paper describes for the first time the isolation and purification of a polar chemical marker known as methyl gallate from Labisia pumila leaves. This compound was isolated and purified through an open column chromatography. Analytical thin layer chromatography (TLC) was used to monitor the separation of the compound. The pure compound obtained was further analyzed using gas chromatography mass spectrometry direct injection probe (GCMS DI-probe) while identification of the structure was performed using nuclear magnetic resonance (NMR) spectroscope by conducting various analysis such as 1H 13C and Heteronuclear Multiple Bond Correlation (HMBC).


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Abstract

This paper describes for the first time the isolation and purification of a polar chemical marker known as methyl gallate from Labisia pumila leaves. This compound was isolated and purified through an open column chromatography. Analytical thin layer chromatography (TLC) was used to monitor the separation of the compound. The pure compound obtained was further analyzed using gas chromatography mass spectrometry direct injection probe (GCMS DI-probe) while identification of the structure was performed using nuclear magnetic resonance (NMR) spectroscope by conducting various analysis such as 1H 13C and Heteronuclear Multiple Bond Correlation (HMBC).

Additional Metadata

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Item Type: Article
AGROVOC Term: Identification
AGROVOC Term: Isolation
AGROVOC Term: Biochemical markers
AGROVOC Term: Purification
AGROVOC Term: Leaves
AGROVOC Term: Purification
AGROVOC Term: Column chromatography
AGROVOC Term: Thin layer chromatography
AGROVOC Term: Separation
AGROVOC Term: Gas chromatography
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:29
URI: http://webagris.upm.edu.my/id/eprint/24519

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