Citation
Zuraida Ab. Rahman, . and Zulkifli Ahmad Seman, . and Habibuddin Hashim, . and Naziah Basirun, . Regeneration of Malaysian rice variety MR 219 viasomaticembryogenesis. pp. 167-177. ISSN 1394-9829
Abstract
Tissue culture plant regeneration protocols were developed for the Malaysian rice variety MR 219 (Oryza sativa L. ssp. indica). Following these protocols 78 of callus became embryogenic when cultured on a medium containing 1 mg/litre 2 4-D (2 4-dichlorophenoxyacetic acid) and 10 mg/litre NAA (a-naphthaleneacetic acid). The highest frequency of somatic embryo initiation (81) was obtained by incubating embryogenic calli on a medium containing 10 mg/litre ABA (abscisic acid) and 9 g/litre gelrite agar for 6 weeks. These embryos regenerated the highest number of plantlets when they were transferred to a medium containing 3 mg/litre kinetin and 0.5 mg/litre NAA. After 8 weeks incubation 9 plantlets per 3 g of somatic embryos were produced. Hence by manipulating plant growth regulators in the culture media one medium was established for each of the following phases namely the induction of embryogenic calli their subculture and the regeneration of plantlets. These protocols can facilitate the production of large numbers of embryogenic calli with high regeneration capacity and maybe incorporated into the genetic transformation system for the rice variety MR 219.
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Abstract
Tissue culture plant regeneration protocols were developed for the Malaysian rice variety MR 219 (Oryza sativa L. ssp. indica). Following these protocols 78 of callus became embryogenic when cultured on a medium containing 1 mg/litre 2 4-D (2 4-dichlorophenoxyacetic acid) and 10 mg/litre NAA (a-naphthaleneacetic acid). The highest frequency of somatic embryo initiation (81) was obtained by incubating embryogenic calli on a medium containing 10 mg/litre ABA (abscisic acid) and 9 g/litre gelrite agar for 6 weeks. These embryos regenerated the highest number of plantlets when they were transferred to a medium containing 3 mg/litre kinetin and 0.5 mg/litre NAA. After 8 weeks incubation 9 plantlets per 3 g of somatic embryos were produced. Hence by manipulating plant growth regulators in the culture media one medium was established for each of the following phases namely the induction of embryogenic calli their subculture and the regeneration of plantlets. These protocols can facilitate the production of large numbers of embryogenic calli with high regeneration capacity and maybe incorporated into the genetic transformation system for the rice variety MR 219.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Rice |
| AGROVOC Term: | Somatic embryogenesis |
| AGROVOC Term: | Regeneration |
| AGROVOC Term: | Oryza sativa |
| AGROVOC Term: | Plantlets (seedlings) |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | Callus |
| AGROVOC Term: | Somatic embryos |
| AGROVOC Term: | Kinetin |
| AGROVOC Term: | Plant growth regulators |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24527 |
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