Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato

Rosnah Hassan, . and Tang Sui Yan, . and Umi Kalsom Md. Shah, . and Sahilah Abd. Mutalib, . and Zaimawati Mohamed Nejis, . and Son Rodu, . Identification and characterization of actinomycetes for biological control of bacterial wilt of Ralstonia solanacearum isolated from tomato. pp. 103-114. ISSN 1394-9829

Five actinomycetes which showed antimicrobial activity towards Ralstonia solanacearum were identified using specific polymerase chain reaction (PCR) of 16S rDNA gene. Strain C1 and Strain G10 were identified as Streptomyces aureofaciens and S. roseoflavus respectively. All actinomycetes were then characterized using antimicrobial and extracellular enzyme activity metabolic and restriction fragment length polymorphism (RFLP) profiles. Strain A3 showed positive reaction to three bacteria namely Xanthomonas campestris Staphylococcus aureus and Listeria monocytogenes. Strain C1 and Strain I15 showed positive reaction towards S. aureus and X. campestris respectively. Strains A3 C1 and I15 were able to metabolize xylan and cellulose while Strain G10 and Strain L8 were able to use all substrates (xylan mannan and cellulose) as carbon sources. All the Streptomyces strains were positive towards more than 25 carbon sources and can be differentiated into five distinct strains. These results were consistent and confirmed with DNA analysis of RFLP profiles. The specific amplification of 16S rDNA PCR restriction profiles for the strains using three restriction endonucleases resulted two restriction profiles produced from the digested 16S rDNA product using HaeIII (H1“H2) and HinfI (Hf1“Hf2) while PstI produced three restriction profiles (P1“P3). No profiles were produced from restriction endonuclases of XbaI SpeI and BamHI.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/38-1/Actinomycetes...

Abstract

Five actinomycetes which showed antimicrobial activity towards Ralstonia solanacearum were identified using specific polymerase chain reaction (PCR) of 16S rDNA gene. Strain C1 and Strain G10 were identified as Streptomyces aureofaciens and S. roseoflavus respectively. All actinomycetes were then characterized using antimicrobial and extracellular enzyme activity metabolic and restriction fragment length polymorphism (RFLP) profiles. Strain A3 showed positive reaction to three bacteria namely Xanthomonas campestris Staphylococcus aureus and Listeria monocytogenes. Strain C1 and Strain I15 showed positive reaction towards S. aureus and X. campestris respectively. Strains A3 C1 and I15 were able to metabolize xylan and cellulose while Strain G10 and Strain L8 were able to use all substrates (xylan mannan and cellulose) as carbon sources. All the Streptomyces strains were positive towards more than 25 carbon sources and can be differentiated into five distinct strains. These results were consistent and confirmed with DNA analysis of RFLP profiles. The specific amplification of 16S rDNA PCR restriction profiles for the strains using three restriction endonucleases resulted two restriction profiles produced from the digested 16S rDNA product using HaeIII (H1“H2) and HinfI (Hf1“Hf2) while PstI produced three restriction profiles (P1“P3). No profiles were produced from restriction endonuclases of XbaI SpeI and BamHI.

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Item Type:	Article
AGROVOC Term:	Ralstonia solanacearum
AGROVOC Term:	Tomatoes
AGROVOC Term:	Wilts
AGROVOC Term:	Bacteria
AGROVOC Term:	Identification
AGROVOC Term:	Actinomycetes
AGROVOC Term:	Biological control
AGROVOC Term:	Antimicrobial properties
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	PCR
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24534

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