Citation
Noraini Samat, . and Shanmugavelu Sithambaram, . and Wong Hee Kum, . and Phong Su Fun, . and Thayalini Kathiraser, . Detection of Lactobacillus Bacteroides and Clostridium perfringens in the gastrointestinal contents of chicken fed different diets by real-time PCR. pp. 81-87. ISSN 1394-9829
Abstract
The rapid molecular method of real-time PCR assay was used to detect the presence of Lactobacillus Bacteroides (including Prevottella-Porphyromonas) and Clostridium perfringens groups from the intestinal contents of 30 samples of chickens fed five different types of diet. All the samples were positive for Lactobacillus and Bacteroides. A total of 21 out of 30 samples were positive for C. perfringens. The DNA sequencing results confirmed the identity of the microorganisms as Lactobacillus salivarius Bacteroides vulgatus Bacteroides caccae Clostridium perfringens and Clostridium kluyveri. These partial DNA sequences of 16S rRNA genes were deposited in the Gene Bank under accession numbers FJ151421 FJ211591 FJ375300 FJ182043 and FJ182042 respectively.
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Abstract
The rapid molecular method of real-time PCR assay was used to detect the presence of Lactobacillus Bacteroides (including Prevottella-Porphyromonas) and Clostridium perfringens groups from the intestinal contents of 30 samples of chickens fed five different types of diet. All the samples were positive for Lactobacillus and Bacteroides. A total of 21 out of 30 samples were positive for C. perfringens. The DNA sequencing results confirmed the identity of the microorganisms as Lactobacillus salivarius Bacteroides vulgatus Bacteroides caccae Clostridium perfringens and Clostridium kluyveri. These partial DNA sequences of 16S rRNA genes were deposited in the Gene Bank under accession numbers FJ151421 FJ211591 FJ375300 FJ182043 and FJ182042 respectively.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Lactobacillus |
| AGROVOC Term: | Bacteroides |
| AGROVOC Term: | Clostridium perfringens |
| AGROVOC Term: | Gastrointestinal system |
| AGROVOC Term: | Animal feeding |
| AGROVOC Term: | Diet preferences |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | DNA sequence |
| AGROVOC Term: | Intestinal microorganisms |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24537 |
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