Detection of Lactobacillus Bacteroides and Clostridium perfringens in the gastrointestinal contents of chicken fed different diets by real-time PCR


Citation

Noraini Samat, . and Shanmugavelu Sithambaram, . and Wong Hee Kum, . and Phong Su Fun, . and Thayalini Kathiraser, . Detection of Lactobacillus Bacteroides and Clostridium perfringens in the gastrointestinal contents of chicken fed different diets by real-time PCR. pp. 81-87. ISSN 1394-9829

Abstract

The rapid molecular method of real-time PCR assay was used to detect the presence of Lactobacillus Bacteroides (including Prevottella-Porphyromonas) and Clostridium perfringens groups from the intestinal contents of 30 samples of chickens fed five different types of diet. All the samples were positive for Lactobacillus and Bacteroides. A total of 21 out of 30 samples were positive for C. perfringens. The DNA sequencing results confirmed the identity of the microorganisms as Lactobacillus salivarius Bacteroides vulgatus Bacteroides caccae Clostridium perfringens and Clostridium kluyveri. These partial DNA sequences of 16S rRNA genes were deposited in the Gene Bank under accession numbers FJ151421 FJ211591 FJ375300 FJ182043 and FJ182042 respectively.


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Abstract

The rapid molecular method of real-time PCR assay was used to detect the presence of Lactobacillus Bacteroides (including Prevottella-Porphyromonas) and Clostridium perfringens groups from the intestinal contents of 30 samples of chickens fed five different types of diet. All the samples were positive for Lactobacillus and Bacteroides. A total of 21 out of 30 samples were positive for C. perfringens. The DNA sequencing results confirmed the identity of the microorganisms as Lactobacillus salivarius Bacteroides vulgatus Bacteroides caccae Clostridium perfringens and Clostridium kluyveri. These partial DNA sequences of 16S rRNA genes were deposited in the Gene Bank under accession numbers FJ151421 FJ211591 FJ375300 FJ182043 and FJ182042 respectively.

Additional Metadata

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Item Type: Article
AGROVOC Term: Lactobacillus
AGROVOC Term: Bacteroides
AGROVOC Term: Clostridium perfringens
AGROVOC Term: Gastrointestinal system
AGROVOC Term: Animal feeding
AGROVOC Term: Diet preferences
AGROVOC Term: PCR
AGROVOC Term: Polymerase chain reaction
AGROVOC Term: DNA sequence
AGROVOC Term: Intestinal microorganisms
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:29
URI: http://webagris.upm.edu.my/id/eprint/24537

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