Analysis of phenolics in Anacardium occidentale shoot extracts using a reversed-phase high performance liquid chromatography tandem mass spectrometry (RP-HPLC-MS)

Chauvin Alan, . and Mohd. Shukri Mohd. Ali, . Analysis of phenolics in Anacardium occidentale shoot extracts using a reversed-phase high performance liquid chromatography tandem mass spectrometry (RP-HPLC-MS). pp. 221-230. ISSN 1394-9829

Two batches of local varieties (red and yellow) of Malaysian traditional vegetables or ulam Anacardium occidentale locally known as gajus collected from MARDI Seberang Perai germplasm were screened for phenolic.compounds. Quantification of the individual phenolic compounds was carried out using a reversed-phase high performance liquid chromatography tandem mass spectrometry (RP-HPLC-MS2) subjected to co-chromatography with standards fragmentation pattern absorbance spectra and elution order. Atotal of 15 phenolic compounds in both varieties mainly flavonol glycosides were identified. The total flavonols were quantified and found to be significantly (p0.05) varied between the red and the yellow varieties (12.1 0.3 mg/g fresh weight fw. in the red variety and 6.4 0.4 mg/g fw. in the yellow variety respectively). Kaempferol-3-O-glucoside is the highest constituent in both varieties followed by kaempferol-3-O arabinofuranoside and quercetin3-O-glucoside. RP-HPLC-MS2 has efficiently assisted the identification and quantification of the phenolic compounds in two varieties of Anacardium occidentale.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/38-2/Anacardium%20...

Abstract

Two batches of local varieties (red and yellow) of Malaysian traditional vegetables or ulam Anacardium occidentale locally known as gajus collected from MARDI Seberang Perai germplasm were screened for phenolic.compounds. Quantification of the individual phenolic compounds was carried out using a reversed-phase high performance liquid chromatography tandem mass spectrometry (RP-HPLC-MS2) subjected to co-chromatography with standards fragmentation pattern absorbance spectra and elution order. Atotal of 15 phenolic compounds in both varieties mainly flavonol glycosides were identified. The total flavonols were quantified and found to be significantly (p0.05) varied between the red and the yellow varieties (12.1 0.3 mg/g fresh weight fw. in the red variety and 6.4 0.4 mg/g fw. in the yellow variety respectively). Kaempferol-3-O-glucoside is the highest constituent in both varieties followed by kaempferol-3-O arabinofuranoside and quercetin3-O-glucoside. RP-HPLC-MS2 has efficiently assisted the identification and quantification of the phenolic compounds in two varieties of Anacardium occidentale.

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Item Type:	Article
AGROVOC Term:	Anacardium occidentale
AGROVOC Term:	Phenolic compounds
AGROVOC Term:	High performance liquid chromatography
AGROVOC Term:	Mass spectrometry
AGROVOC Term:	Germplasm
AGROVOC Term:	Flavonols
AGROVOC Term:	Glycosides
AGROVOC Term:	Weight
AGROVOC Term:	Extraction
AGROVOC Term:	Centrifugation
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24550

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