Screening for cellulase activities in actinomycetes isolated from different locations of Peninsular Malaysia


Citation

Lim Jeffrey Seng Heng, . and Azrizal Mohd Rashidi, . Screening for cellulase activities in actinomycetes isolated from different locations of Peninsular Malaysia. pp. 153-157. ISSN 1394-9829

Abstract

A total of 282 isolates of actinomycetes were isolated from soil samples collected from few selected areas in Peninsular Malaysia (Selangor Johor and Kuala Lumpur). These actinomycetes were observed to have different colony colours ranging from dark grey (42.6) yellowish white (7.4) greyish white (40.8) white (7.8) reddish (0.3) and brownish (1.1). All the isolates were screened for their cellulase activity which 37.6 (106 isolates) gave positive results. Isolate number 153 159 177 184 185 191 196 200 and 262 which produced halo zone of 15.0“23.0 mm were identified by targeting their 16S-rRNA sequence. The results obtained showed that all the isolates belong to the genus Streptomyces spp.


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Abstract

A total of 282 isolates of actinomycetes were isolated from soil samples collected from few selected areas in Peninsular Malaysia (Selangor Johor and Kuala Lumpur). These actinomycetes were observed to have different colony colours ranging from dark grey (42.6) yellowish white (7.4) greyish white (40.8) white (7.8) reddish (0.3) and brownish (1.1). All the isolates were screened for their cellulase activity which 37.6 (106 isolates) gave positive results. Isolate number 153 159 177 184 185 191 196 200 and 262 which produced halo zone of 15.0“23.0 mm were identified by targeting their 16S-rRNA sequence. The results obtained showed that all the isolates belong to the genus Streptomyces spp.

Additional Metadata

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Item Type: Article
AGROVOC Term: Actinomycetes
AGROVOC Term: Cellulase
AGROVOC Term: Isolation
AGROVOC Term: Screening tests
AGROVOC Term: Soil sampling
AGROVOC Term: Colony counting
AGROVOC Term: Streptomyces
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:29
URI: http://webagris.upm.edu.my/id/eprint/24584

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