Optimization of submerged culture for the production of Lentinulaedodes mycelia biomass and amino acid composition by different temperatures


Citation

Madzlan Kasran, . and Mohd Khan Ayob, . and Suri Roowi, . and Aminuddin Hussin, . and Abidin Hamid, . and Mohamad Kamal Abd. Kadir, . Optimization of submerged culture for the production of Lentinulaedodes mycelia biomass and amino acid composition by different temperatures. pp. 131-138. ISSN 1394-9829

Abstract

Lentinula edodes and its mycelium are known for their unique properties and have been used in food and medicinal industry for centuries. The effect of different temperatures and medium conditions on yield of dry mycelium and total amino acid content was studied. Submerged culture technique was employed to grow mycelium in PDB (potato dextrose broth) and PDB (PDB yeast extract malt extract peptone KH2PO4 and MgSO4.7H2O) media under different temperatures. The amino acid composition of the mycelium was determined by the High Performance Liquid Chromatography techniques (HPLC). An optimum growth of the mycelium was observed on the PDB medium incubated at 25 C. The mycelium harvested had the highest content of amino acid of which the major component was glutamic acid.


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Abstract

Lentinula edodes and its mycelium are known for their unique properties and have been used in food and medicinal industry for centuries. The effect of different temperatures and medium conditions on yield of dry mycelium and total amino acid content was studied. Submerged culture technique was employed to grow mycelium in PDB (potato dextrose broth) and PDB (PDB yeast extract malt extract peptone KH2PO4 and MgSO4.7H2O) media under different temperatures. The amino acid composition of the mycelium was determined by the High Performance Liquid Chromatography techniques (HPLC). An optimum growth of the mycelium was observed on the PDB medium incubated at 25 C. The mycelium harvested had the highest content of amino acid of which the major component was glutamic acid.

Additional Metadata

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Item Type: Article
AGROVOC Term: Lentinula edodes
AGROVOC Term: Biomass
AGROVOC Term: Amino acids
AGROVOC Term: Optimization methods
AGROVOC Term: Temperature
AGROVOC Term: Mycelium
AGROVOC Term: High performance liquid chromatography
AGROVOC Term: HPLC
AGROVOC Term: Glutamic acid
AGROVOC Term: Basidiomycetes
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:29
URI: http://webagris.upm.edu.my/id/eprint/24586

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