Correlation of microspore nuclear development with male inflorescence morphology in Elaeis oleifera Elaeis guineensis and the OxG hybrid

Marhalil Marjuni, . and Noorhariza Mohd Zaki, . and Che Radziah Che Mohd Zain, . and M. A. Latif, . and Nordiana Hanim Mohd Nor, . and Maria Madon, . Correlation of microspore nuclear development with male inflorescence morphology in Elaeis oleifera Elaeis guineensis and the OxG hybrid. pp. 104-108. ISSN 1511-2780

Microspore nuclear stages of the Elaeis species and their OxG hybrid were examined and correlated with the respective male inflorescence morphology. Isolated pollen mother cells (PMC) and microspores obtained from squashed male flower buds were stained with blue fluorescent 4 6-diamidino-2-phenylindole (DAPI) dye and observed with the Zeiss Axioplan epifluorescent microscope to determine their nuclear stages. The male inflorescence from both the Elaeis species and the (OxG) hybrid with a perfectly closed spathe having whitish inflorescences and friable individual spikelets indicated that PMC activities were ongoing within the anthers of male flower buds. In E. guineensis the whitish pale yellow or light green spikelets with flexible texture obtained from a perfectly closed spathe appeared to be morphological markers for uninucleate microspores. For E. oleifera inflorescences the colour varies from pale beige to umber brown with flexible spikelets while the spathe varied from being perfectly closed to slightly open. The observed morphology indicated the presence of uninucleate microspores in E. oleifera. For the OxG hybrid morphological markers resembled that of E. oleifera. By staining a plethora of male flower buds from various oil palm male inflorescences the meiosis and mitosis phases were also profiled.

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Abstract

Microspore nuclear stages of the Elaeis species and their OxG hybrid were examined and correlated with the respective male inflorescence morphology. Isolated pollen mother cells (PMC) and microspores obtained from squashed male flower buds were stained with blue fluorescent 4 6-diamidino-2-phenylindole (DAPI) dye and observed with the Zeiss Axioplan epifluorescent microscope to determine their nuclear stages. The male inflorescence from both the Elaeis species and the (OxG) hybrid with a perfectly closed spathe having whitish inflorescences and friable individual spikelets indicated that PMC activities were ongoing within the anthers of male flower buds. In E. guineensis the whitish pale yellow or light green spikelets with flexible texture obtained from a perfectly closed spathe appeared to be morphological markers for uninucleate microspores. For E. oleifera inflorescences the colour varies from pale beige to umber brown with flexible spikelets while the spathe varied from being perfectly closed to slightly open. The observed morphology indicated the presence of uninucleate microspores in E. oleifera. For the OxG hybrid morphological markers resembled that of E. oleifera. By staining a plethora of male flower buds from various oil palm male inflorescences the meiosis and mitosis phases were also profiled.

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Item Type:	Article
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Elaeis oleifera
AGROVOC Term:	Microspores (pollen)
AGROVOC Term:	Inflorescences
AGROVOC Term:	morphology
AGROVOC Term:	Hybrids
AGROVOC Term:	Pollen
AGROVOC Term:	Buds
AGROVOC Term:	Meiosis
AGROVOC Term:	Mitosis
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24625

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