Citation
Abdul Masani Mat Yunus, . and Rafiqah Md Aman, . and Omar Abdul Rasid, . and Ahmad Parveez Ghulam Kadir, . and Nurfahisza Abdul Rahman, . and Ismanizan Ismail, . and Nor Hanin Ayub, . Molecular analysis of transgenic oil palm to detect the presence of transgenes. pp. 96-103. ISSN 1511-2780
Abstract
In order to meet the increasing demand of oleochemical industries genetic engineering was initiated to modify the fatty acid composition of oil palm with the aim to increase the production of oleic acid to be used as a feedstock. Biolistic approach was used for inserting an antisense palmitoyl-ACP-thioesterase sequence into oil palm tissues to down regulate the activity of palmitoyl-ACP-thioesterase and increase the accumulation of oleic acid at the expense of palmitic acid. Genomic DNA was isolated from transgenic oil palm and polymerase chain reaction (PCR) amplification of the selectable marker gene (bar) and gene of interest antisense palmitoyl-ACP-thioesterase was successfully carried out. The presence of transgenes was confirmed by DNA sequencing.
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Abstract
In order to meet the increasing demand of oleochemical industries genetic engineering was initiated to modify the fatty acid composition of oil palm with the aim to increase the production of oleic acid to be used as a feedstock. Biolistic approach was used for inserting an antisense palmitoyl-ACP-thioesterase sequence into oil palm tissues to down regulate the activity of palmitoyl-ACP-thioesterase and increase the accumulation of oleic acid at the expense of palmitic acid. Genomic DNA was isolated from transgenic oil palm and polymerase chain reaction (PCR) amplification of the selectable marker gene (bar) and gene of interest antisense palmitoyl-ACP-thioesterase was successfully carried out. The presence of transgenes was confirmed by DNA sequencing.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Oil palm |
| AGROVOC Term: | Transgenesis |
| AGROVOC Term: | Transgenics |
| AGROVOC Term: | Genetic engineering |
| AGROVOC Term: | Fatty acids |
| AGROVOC Term: | Biolistics |
| AGROVOC Term: | Oleic acid |
| AGROVOC Term: | Plant tissues |
| AGROVOC Term: | Palmitic acid |
| AGROVOC Term: | genomics |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24626 |
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