Optimisation of enzymatic sludge palm oil recovery from palm oil mill effluent using response surface methodology

Nor Faizah Jalani, . and Astimar Abdul Aziz, . and Mohamadiah Banjari, . and Noorshamsiana A. W., . and Nor Hayati M., . and Norhayati S., . Optimisation of enzymatic sludge palm oil recovery from palm oil mill effluent using response surface methodology. pp. 348-356. ISSN 1511-2780

Various operational variables affecting the extraction of sludge palm oil (SPO) from palm oil mill effluent (POME) by enzymatic treatment were investigated. The enzyme used in the study was cellulase from Trichoderma reseei commercially known as Celluclast 1.5L FG (Novozymes). A software application of the Central Composite Face-centred Design (CCFD) known as Response Surface Methodology (RSM) was used to study the effects of independent variables generated by the Design Expert Software namely incubation time X1 (12.73-147.27 hr) enzyme concentration X2 (0.08-0.92) and rotation speed X3 (65.91-234.09 rpm) on the oil recovered from POME. In this study a second-order polynomial regression model was used to interpret the experimental data with a coefficient of determination (R2) value of 0.852. From the RSM-generated model the optimum conditions for extraction of oil from POME were identified to be at an enzyme concentration value of 0.25 in 81.46 hr reaction time with a rotation speed of 111.05 rpm. Predicted oil yield was 93.56 while the experimental oil yield was 81.95 as revealed by the One Sample T-Test of confirmatory studies.

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Abstract

Various operational variables affecting the extraction of sludge palm oil (SPO) from palm oil mill effluent (POME) by enzymatic treatment were investigated. The enzyme used in the study was cellulase from Trichoderma reseei commercially known as Celluclast 1.5L FG (Novozymes). A software application of the Central Composite Face-centred Design (CCFD) known as Response Surface Methodology (RSM) was used to study the effects of independent variables generated by the Design Expert Software namely incubation time X1 (12.73-147.27 hr) enzyme concentration X2 (0.08-0.92) and rotation speed X3 (65.91-234.09 rpm) on the oil recovered from POME. In this study a second-order polynomial regression model was used to interpret the experimental data with a coefficient of determination (R2) value of 0.852. From the RSM-generated model the optimum conditions for extraction of oil from POME were identified to be at an enzyme concentration value of 0.25 in 81.46 hr reaction time with a rotation speed of 111.05 rpm. Predicted oil yield was 93.56 while the experimental oil yield was 81.95 as revealed by the One Sample T-Test of confirmatory studies.

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Item Type:	Article
AGROVOC Term:	Palm oils
AGROVOC Term:	Mills
AGROVOC Term:	Effluents
AGROVOC Term:	Cellulase
AGROVOC Term:	Yields
AGROVOC Term:	Trichoderma reesei
AGROVOC Term:	Enzymes
AGROVOC Term:	Citric acid
AGROVOC Term:	Enzymatic hydrolysis
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24635

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