Citation
Ho C. L., . and Ooi S. E., . and Chin C. F., . and Norashikin Sarpan, . and Ong-Abdullah Meilina, . and Parameswari Namasivayam, . Representational difference analysis (RDA) for the identification of DNA markers associated with tissue culture amenity in oil palm. pp. 305-313. ISSN 1511-2780
Abstract
Tissue culture is a promising technology for mass propagation of high yielding oil palm. However the large scale production of clonal palm is still not optimal. The application of DNA marker for early diagnosis of high quality ortets (selected oil palm tree for cloning) would greatly help in reducing the production cost. Representational difference analysis (RDA) was used to identify genomic differences between the oil palm explant tissues of the same genotypes but producing different somatic embryogenesis rates in culture. The putative RDA products isolated were cloned sequenced and verified. Among the products isolated C1-20 and C14-19 from forward and reverse RDA respectively possess a single nucleotide change when aligned with the equivalent DNA region of the subtracted tissues. However further verification is needed to confirm the discriminating effects of these products on the fecundity of the selected ortets.
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Abstract
Tissue culture is a promising technology for mass propagation of high yielding oil palm. However the large scale production of clonal palm is still not optimal. The application of DNA marker for early diagnosis of high quality ortets (selected oil palm tree for cloning) would greatly help in reducing the production cost. Representational difference analysis (RDA) was used to identify genomic differences between the oil palm explant tissues of the same genotypes but producing different somatic embryogenesis rates in culture. The putative RDA products isolated were cloned sequenced and verified. Among the products isolated C1-20 and C14-19 from forward and reverse RDA respectively possess a single nucleotide change when aligned with the equivalent DNA region of the subtracted tissues. However further verification is needed to confirm the discriminating effects of these products on the fecundity of the selected ortets.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Oil palm |
| AGROVOC Term: | Molecular markers |
| AGROVOC Term: | DNA |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | Plant propagation |
| AGROVOC Term: | Clonal variation |
| AGROVOC Term: | Cloning |
| AGROVOC Term: | Production costs |
| AGROVOC Term: | genomics |
| AGROVOC Term: | Nucleotides |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24638 |
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