Citation
Khattab A. A., . and Ibrahim M. I. M., . and El-Kady A. A., . Ochratoxin A biosorption onto genetically improved of Lactobacillus delbrueckii mutants. pp. 515-522. ISSN 2231-754
Abstract
Eight lactic acid bacterial strains were evaluated for Ochratoxin A (OTA) biosorption. Results showed that Lactobacillus delbrueckii NRRL B-1024 is the most effective strain for OTA biosorption of 50 ng/ml within one hour. It was selected and mutagenized using ethyl methanesulfonate (EMS) to induce genetic variations in relation to improve OTA biosorption. Seven of 19 mutants exhibited OTA biosorption higher than the wild-type strain. The maximum capacity was achieved using the mutant EMS-9 which recorded 82.62 ng/ml of OTA uptake within one hour. The mutant EMS-9 appears to be the most superior strain for OTA biosorption since 88 of the initial OTA concentration was removed using 40 (v/v) inoculum concentration within one hour after studying the effect of inoculum concentration on OTA uptake. Finally the application of the Random Amplified Polymorphic DNA (RAPD) technique by polymerase chain reaction (PCR) on some selected mutants lead to correlate the genetic characteristics of the mutants with the results obtained from the biosorption process in comparison with the wild type strain. Differences in RAPD patterns confirmed the evidence of genetic variability induced in Lb. delbrueckii NRRL B-1024 genome after EMS-treatment.
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Abstract
Eight lactic acid bacterial strains were evaluated for Ochratoxin A (OTA) biosorption. Results showed that Lactobacillus delbrueckii NRRL B-1024 is the most effective strain for OTA biosorption of 50 ng/ml within one hour. It was selected and mutagenized using ethyl methanesulfonate (EMS) to induce genetic variations in relation to improve OTA biosorption. Seven of 19 mutants exhibited OTA biosorption higher than the wild-type strain. The maximum capacity was achieved using the mutant EMS-9 which recorded 82.62 ng/ml of OTA uptake within one hour. The mutant EMS-9 appears to be the most superior strain for OTA biosorption since 88 of the initial OTA concentration was removed using 40 (v/v) inoculum concentration within one hour after studying the effect of inoculum concentration on OTA uptake. Finally the application of the Random Amplified Polymorphic DNA (RAPD) technique by polymerase chain reaction (PCR) on some selected mutants lead to correlate the genetic characteristics of the mutants with the results obtained from the biosorption process in comparison with the wild type strain. Differences in RAPD patterns confirmed the evidence of genetic variability induced in Lb. delbrueckii NRRL B-1024 genome after EMS-treatment.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Lactobacillus delbrueckii |
| AGROVOC Term: | Mutants |
| AGROVOC Term: | Lactic acid bacteria |
| AGROVOC Term: | Ochratoxins |
| AGROVOC Term: | Mutagenesis |
| AGROVOC Term: | Sorption |
| AGROVOC Term: | Biosorption (bioremediation) |
| AGROVOC Term: | Random amplified polymorphic DNA |
| AGROVOC Term: | Polymerase chain reaction |
| AGROVOC Term: | Genetic variation |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24673 |
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