Isolation characterization and screening of folate-producing bacteria from traditional fermented food (dadih)

Utami T., . and Purwandhani S. N., . and Millati R., . and Rahayu E. S., . Isolation characterization and screening of folate-producing bacteria from traditional fermented food (dadih). pp. 566-572. ISSN 2231-7546

Folate represents an important B vitamin participating in one-carbon transfer reaction required in many metabolic pathways especially purine and pyrimidine biosynthesis which indicates the importance of folate in human metabolism. Fermented milk products using lactic acid bacteria (LAB) are good sources of such vitamins. In order to find suitable strains capable producing high folate isolation and characterization of LAB from traditional fermented milk (dadih) were carried out. The isolated bacteria were characterized biochemically phenotypically genetically and were screened for their ability to produce folate during fermentation in skim milk. Phenotypic characterization was performed using API 50 CHL; genotypic characterization was conducted based on the sequence of 16S rRNA genes while the determination of folate level was done using Vita fast folate kit. From this study 17 isolates from dadih were obtained and based on phenotypic and genotypic 16 of them were identified as Lactobacillus plantarum. Folate production of the 17 selected isolates was between 12.43 3.13 to 27.84 5.80 g/L and the folate production of Lactobacillus plantarum Dad-13 as the control was 29.27 3.91 g/L.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/25%20(02)%202018/(16).p...

Abstract

Folate represents an important B vitamin participating in one-carbon transfer reaction required in many metabolic pathways especially purine and pyrimidine biosynthesis which indicates the importance of folate in human metabolism. Fermented milk products using lactic acid bacteria (LAB) are good sources of such vitamins. In order to find suitable strains capable producing high folate isolation and characterization of LAB from traditional fermented milk (dadih) were carried out. The isolated bacteria were characterized biochemically phenotypically genetically and were screened for their ability to produce folate during fermentation in skim milk. Phenotypic characterization was performed using API 50 CHL; genotypic characterization was conducted based on the sequence of 16S rRNA genes while the determination of folate level was done using Vita fast folate kit. From this study 17 isolates from dadih were obtained and based on phenotypic and genotypic 16 of them were identified as Lactobacillus plantarum. Folate production of the 17 selected isolates was between 12.43 3.13 to 27.84 5.80 g/L and the folate production of Lactobacillus plantarum Dad-13 as the control was 29.27 3.91 g/L.

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Item Type:	Article
AGROVOC Term:	Fermented milk
AGROVOC Term:	Lactic acid bacteria
AGROVOC Term:	Vitamins B
AGROVOC Term:	Skim milk
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Biochemical indicators (organisms)
AGROVOC Term:	Genetically modified bacteria
AGROVOC Term:	Molecular biology
AGROVOC Term:	Phenotypes
AGROVOC Term:	Lactobacillus plantarum
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24681

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