Citation
Chung H. H., . Real-time polymerase chain reaction (RT-PCR) for the authentication of raw meats. pp. 632-638. ISSN 2231-7546
Abstract
Meat adulteration has been a significant issue in todays food industry as it intertwine with religious social and economic values. PCR based techniques for the detection of meat species in a meat admixture are primarily used by the industry as a reliable approach due to its sensitivity and reliability. This paper describes the design and verification of real-time polymerase chain reaction (RT-PCR) based assay for the detection of meat from various nontarget species by using species specific oligonucleotides. Five sets of species-specific primers have been developed to target small regions ( 150 bp) of the mitochondrial D-loop. The specificity sensitivity and reliability of each assay have been verified by using SYBR Green based RT-PCR. By using a cut-off CT of 30 cycles all assays show sensitivity down to 0.05 of the DNA spike level. When applied to DNA templates from raw meat admixtures assays were able to detect the target species up to a level of 0.1. Hence this verify the potential applicability of these assays in the meat industry.
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Abstract
Meat adulteration has been a significant issue in todays food industry as it intertwine with religious social and economic values. PCR based techniques for the detection of meat species in a meat admixture are primarily used by the industry as a reliable approach due to its sensitivity and reliability. This paper describes the design and verification of real-time polymerase chain reaction (RT-PCR) based assay for the detection of meat from various nontarget species by using species specific oligonucleotides. Five sets of species-specific primers have been developed to target small regions ( 150 bp) of the mitochondrial D-loop. The specificity sensitivity and reliability of each assay have been verified by using SYBR Green based RT-PCR. By using a cut-off CT of 30 cycles all assays show sensitivity down to 0.05 of the DNA spike level. When applied to DNA templates from raw meat admixtures assays were able to detect the target species up to a level of 0.1. Hence this verify the potential applicability of these assays in the meat industry.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Meat |
| AGROVOC Term: | Bos taurus |
| AGROVOC Term: | Pork |
| AGROVOC Term: | Buffalo meat |
| AGROVOC Term: | Chicken meat |
| AGROVOC Term: | Turkey meat |
| AGROVOC Term: | Sus scrofa |
| AGROVOC Term: | Gallus |
| AGROVOC Term: | Cattle |
| AGROVOC Term: | Polymerase chain reaction |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24689 |
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