Citation
Rosland Abel Stasha Eleanor, . and Soh Kheang Loh, . Process optimisation of 1 3 -propanediol production by Klebsiella pneumoniae strain. pp. 529-536. ISSN 1511-2780
Abstract
The 1 3-propanediol (1 3-PD) is a versatile chemical feedstock with a wide range of applications: cosmetic food and polymers synthesis. Although 1 3-PD is conventionally chemically synthesised it has drawbacks such as requiring high pressure and temperature. Therefore a more economical biological approach to produce 1 3-PD is crucial. This study attempted to investigate the effects of common fermentation parameters “ temperature pH glycerol concentration and inoculum size on the production of 1 3-PD by Klebsiella pneumoniae via one-factor-at-a-time (OFAT) method. The optimised conditions: temperature 34C; pH 7.5; glycerol concentration 30 g litre-1 and inoculum size 20 (v/v) showed 1.78-fold increase in 1 3-PD production i.e. from the initial 4.89 g litre-1 to 8.70 g litre-1 in shake flask experiment after 48 hr. The 1 3- PD productivity by K. pneumoniae was higher (0.18 g litre-1 hr-1) from the unoptimised conditions (0.07 g litre-1 hr-1). The other parameter studied “ varying nitrogen sources in the fermentation medium “ revealed that their addition unexpectedly did not show any improvement in 1 3-PD production unlike those reported in the literature. It can be concluded that both glycerol concentration and incubation temperature were the most significant factors in producing higher 1 3-PD yield.
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Abstract
The 1 3-propanediol (1 3-PD) is a versatile chemical feedstock with a wide range of applications: cosmetic food and polymers synthesis. Although 1 3-PD is conventionally chemically synthesised it has drawbacks such as requiring high pressure and temperature. Therefore a more economical biological approach to produce 1 3-PD is crucial. This study attempted to investigate the effects of common fermentation parameters “ temperature pH glycerol concentration and inoculum size on the production of 1 3-PD by Klebsiella pneumoniae via one-factor-at-a-time (OFAT) method. The optimised conditions: temperature 34C; pH 7.5; glycerol concentration 30 g litre-1 and inoculum size 20 (v/v) showed 1.78-fold increase in 1 3-PD production i.e. from the initial 4.89 g litre-1 to 8.70 g litre-1 in shake flask experiment after 48 hr. The 1 3- PD productivity by K. pneumoniae was higher (0.18 g litre-1 hr-1) from the unoptimised conditions (0.07 g litre-1 hr-1). The other parameter studied “ varying nitrogen sources in the fermentation medium “ revealed that their addition unexpectedly did not show any improvement in 1 3-PD production unlike those reported in the literature. It can be concluded that both glycerol concentration and incubation temperature were the most significant factors in producing higher 1 3-PD yield.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Klebsiella pneumoniae |
| AGROVOC Term: | Chemical compounds |
| AGROVOC Term: | Yeast extracts |
| AGROVOC Term: | Glycerol |
| AGROVOC Term: | Agar |
| AGROVOC Term: | Starter cultures |
| AGROVOC Term: | Cultivation |
| AGROVOC Term: | Fermentation |
| AGROVOC Term: | Analytical methods |
| AGROVOC Term: | High performance liquid chromatography |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24699 |
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