In vitro mass multiplication of Artocarpus heterophyllus Lam var. Tekam yellow

Nurul Husna Mustafa Kamal, . and Maheran Abd Aziz, . and Saleh Kadzimin, . and Azmi Abdul Rashid, . In vitro mass multiplication of Artocarpus heterophyllus Lam var. Tekam yellow. pp. 1289-1314. ISSN 1511-3701

A protocol for rapid micropropagation of Artocarpus heterophyllusfrom seeds of a single fruit was established. The seeds were successfully sterilised using 40 Clorox (20 min) 20 Clorox (15 min) and 50 Clorox (20 min) 20 Clorox (15 min). The survivability percentage was 44.44 while the contamination percentage was 14.81. Experiments to assess the effect of shoot tip and different node positions on shoot induction and to test the effect of decapitation on shoot proliferation were performed. The explants used for both experiments were derived from 8-week-old seedlings grown in half-strength MS basal media supplemented with 2.5 mg/L BAP. There was no significant difference in terms of percentage of explants regenerating shoots and mean shoot number produced per explants. However node 2 significantly produced the highest mean shoot length (2.53 cm). In the decapitation experiment there was no significant difference in terms of percentage of explants regenerating shoots and mean shoot length. Nevertheless decapitated shoots significantly produced the highest mean shoot number per explant (18.33). 2.5 mg/L BAP was chosen as the best treatment for shoot induction from seed with a mean shoot number of 7.33 and mean shoot length of 2.95 cm. For shoot multiplication 1.0 mg/L BAP significantly produced the highest mean shoot number (17.13) while 5.0 mg/L BAP significantly produced the highest shoot length (2.95 cm). For rooting IBA at 2.5 mg/L and 5.0 mg/L produced the highest mean root number at 18.73 and 17.27 respectively. The highest mean root length (3.37 cm) was significantly obtained in the control treatment. The plantlets were successfully acclimatised in a potting mixture consisting of top soil and organic soil (1:1) with 88.89 survival rate.

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Abstract

A protocol for rapid micropropagation of Artocarpus heterophyllusfrom seeds of a single fruit was established. The seeds were successfully sterilised using 40 Clorox (20 min) 20 Clorox (15 min) and 50 Clorox (20 min) 20 Clorox (15 min). The survivability percentage was 44.44 while the contamination percentage was 14.81. Experiments to assess the effect of shoot tip and different node positions on shoot induction and to test the effect of decapitation on shoot proliferation were performed. The explants used for both experiments were derived from 8-week-old seedlings grown in half-strength MS basal media supplemented with 2.5 mg/L BAP. There was no significant difference in terms of percentage of explants regenerating shoots and mean shoot number produced per explants. However node 2 significantly produced the highest mean shoot length (2.53 cm). In the decapitation experiment there was no significant difference in terms of percentage of explants regenerating shoots and mean shoot length. Nevertheless decapitated shoots significantly produced the highest mean shoot number per explant (18.33). 2.5 mg/L BAP was chosen as the best treatment for shoot induction from seed with a mean shoot number of 7.33 and mean shoot length of 2.95 cm. For shoot multiplication 1.0 mg/L BAP significantly produced the highest mean shoot number (17.13) while 5.0 mg/L BAP significantly produced the highest shoot length (2.95 cm). For rooting IBA at 2.5 mg/L and 5.0 mg/L produced the highest mean root number at 18.73 and 17.27 respectively. The highest mean root length (3.37 cm) was significantly obtained in the control treatment. The plantlets were successfully acclimatised in a potting mixture consisting of top soil and organic soil (1:1) with 88.89 survival rate.

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Item Type:	Article
AGROVOC Term:	Artocarpus heterophyllus
AGROVOC Term:	Moraceae
AGROVOC Term:	Jackfruit (tree)
AGROVOC Term:	Seeds
AGROVOC Term:	Micropropagation
AGROVOC Term:	Explants
AGROVOC Term:	Shoot tip culture
AGROVOC Term:	Plant multiplication
AGROVOC Term:	Root inoculation
AGROVOC Term:	Acclimatization
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24816

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