Phylogenetic and expression of ATP-Binding Cassette Transporter genes in Rasbora sarawakensis

Mohd Zulfazli Moktar, . and Balinu Clarissa Patrick, . and Yen Tan Say, . and Abdul Qawiem Jumaan, . and Kit Lim Leonard Whye, . and Ying Tan Hui, . and Hui Chung Hung, . and Robert Arin Vynona, . and Aimi Wahidah Aminan, . and Badiozaman Sulaiman, . Phylogenetic and expression of ATP-Binding Cassette Transporter genes in Rasbora sarawakensis. pp. 1341-1354. ISSN 1511-3701

The ATP-Binding Cassette transporters (ABC transporters) function in various physiological activity allowing vertebrate to thrive even in polluted environment. The objective of this study is to discover ABC genes in Rasbora sarawakensis a species endemic to Borneo and to understand the respective genes regulation. In this research nine gene partial transcripts were isolated via RT-PCR and cloning approaches. Our study showed that most gene transcripts identified share high identities with conserved motif distributions across family. Further phylogenetic analysis revealed a clear divergence into three major functional clades (A2 E1 F1; D2; B4 B8 C2 G2). Expression profiles in six tissues (i.e. brain eye gill intestine muscle and skin) revealed divergence that shed light on tissue-specific gene functional specialization with highlight on B4 B8 and E1 which are expressed in all six organs. The brain and eye were also found to express all ABC genes selected in this study. In conclusion nucleotide profiles of these genes are comparable to the phylogenetic analysis and expression patterns across family. This study implies that an alternative vertebrate model organism can possibly complement the current zebrafish researches.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/Pertanika%20PAPERS...

Abstract

The ATP-Binding Cassette transporters (ABC transporters) function in various physiological activity allowing vertebrate to thrive even in polluted environment. The objective of this study is to discover ABC genes in Rasbora sarawakensis a species endemic to Borneo and to understand the respective genes regulation. In this research nine gene partial transcripts were isolated via RT-PCR and cloning approaches. Our study showed that most gene transcripts identified share high identities with conserved motif distributions across family. Further phylogenetic analysis revealed a clear divergence into three major functional clades (A2 E1 F1; D2; B4 B8 C2 G2). Expression profiles in six tissues (i.e. brain eye gill intestine muscle and skin) revealed divergence that shed light on tissue-specific gene functional specialization with highlight on B4 B8 and E1 which are expressed in all six organs. The brain and eye were also found to express all ABC genes selected in this study. In conclusion nucleotide profiles of these genes are comparable to the phylogenetic analysis and expression patterns across family. This study implies that an alternative vertebrate model organism can possibly complement the current zebrafish researches.

[error in script]

Item Type:	Article
AGROVOC Term:	Fishes
AGROVOC Term:	Cyprinidae
AGROVOC Term:	Endemic species
AGROVOC Term:	RNA sequence
AGROVOC Term:	ATP
AGROVOC Term:	Phylogeny
AGROVOC Term:	Gene expression
AGROVOC Term:	Isolation
AGROVOC Term:	Nucleotide sequence
AGROVOC Term:	Species differences
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24819

Actions (login required)

View Item