Isolating and characterising chitinolytic thermophilic bacteria from Cangar Hot Spring East Java


Citation

Tapatfeto Aime Clorinda, . and Verina Devi, . and Patjajani Tjandra, . and Wahjudi Mariana, . and Chrisnasari Ruth, . and Marianti Purwanto Maria Goretti, . and Pranata Stefan, . Isolating and characterising chitinolytic thermophilic bacteria from Cangar Hot Spring East Java. pp. 1437-1448. ISSN 1511-3701

Abstract

In the present study chitinolytic thermophilic bacteria were collected from Cangar hot spring East Java Indonesia and screened. The 16S rRNA gene sequencing was used to identify the isolated bacterium which showed highest chitinolytic activity. The identified isolate was then characterised based on morphological and physiological analyses. The results showed the isolated bacterium belonged to Bacillus licheniformis. This isolate produced large amounts of chitinase on 0.9 (w/v) colloidal chitin (pH 7.0) at 52C in a very short time (24 hours). Two pairs of primer were designed to detect the presence of glycosyl hydrolase (GH) 18 chitin domain sequences in the isolated bacterium. Two amplicons sized 250 bp and 1000 bp were obtained from PCR process. Then the amplicons were sequenced and analysed. The sequencing results showed the isolated Bacillus licheniformis was proven to have genes encoding ChiA and ChiC domain.


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Abstract

In the present study chitinolytic thermophilic bacteria were collected from Cangar hot spring East Java Indonesia and screened. The 16S rRNA gene sequencing was used to identify the isolated bacterium which showed highest chitinolytic activity. The identified isolate was then characterised based on morphological and physiological analyses. The results showed the isolated bacterium belonged to Bacillus licheniformis. This isolate produced large amounts of chitinase on 0.9 (w/v) colloidal chitin (pH 7.0) at 52C in a very short time (24 hours). Two pairs of primer were designed to detect the presence of glycosyl hydrolase (GH) 18 chitin domain sequences in the isolated bacterium. Two amplicons sized 250 bp and 1000 bp were obtained from PCR process. Then the amplicons were sequenced and analysed. The sequencing results showed the isolated Bacillus licheniformis was proven to have genes encoding ChiA and ChiC domain.

Additional Metadata

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Item Type: Article
AGROVOC Term: Bacillus licheniformis
AGROVOC Term: Bacteria
AGROVOC Term: Thermophilic bacteria
AGROVOC Term: Isolation of microorganisms
AGROVOC Term: Gene sequence
AGROVOC Term: Chitinase
AGROVOC Term: Molecular biology
AGROVOC Term: morphology
AGROVOC Term: Animal physiology
AGROVOC Term: Colloidal properties
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:29
URI: http://webagris.upm.edu.my/id/eprint/24827

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