The response of TLR3 and IL-1 genes following exposure to LPS Poly (I:C) Zymosan in culture of gouramy (Osphronemus gouramy) kidney cells

Kusumawaty Diah, . and Suhandono Sony, . and Pugeg Aryantha I Nyoman, . and Pancoro Adi, . The response of TLR3 and IL-1 genes following exposure to LPS Poly (I:C) Zymosan in culture of gouramy (Osphronemus gouramy) kidney cells. pp. 1479-1488. ISSN 1511-3701

The aims of the present study are to isolate and characterise structure TLR3 and IL1 gene and evaluate the potential and signaling mechanism following exposure of Polyinosinic: polycytidylic acid (poly (I:C)) Lipopolysaccharide (LPS) and Zymosan as antigens in gouramy (Osphronemus gouramy). Gouramy kidney cells were stimulated with LPS Poly I:C and Zymosan. Following incubation at 28C relative expression levels of Toll Like Receptor 3 (TLR3) and interleukin-1 (IL-1) were examined at one hour and six hour after treatment. A Real Time Polimerase Chain Reaction approach was utilised to search for the effects of Poly I:C LPS and Zymosan exposure to gouramy kidney cells between one hour to six hours after treatment LPS in kidney cell increased expression of interleukin-1 and downregulated expression of TLR3. Poly (I:C) which is an antigen that responds to antiviral induces an increase in the transcription both of Toll like receptor 3 and IL-1. Zymosan in kidney cells increased expression of TLR3 but downregulated expression of IL1. This study shows that TLR3 was activated not only by Poly I: C but also LPS and Zymosan. However antigen Poly IC-induced labor IL-1 and TLR3 expressed higher than the antigen LPS and Zymosan so this research showed a TLR3 response to Poly I:C is more dominant than Zymosan and LPS. TLR3 in gouramy kidney cell transcript was identified in response to poly I:C Zymosan and LPS. These results indicate that TLR3 in gouramy also plays a role for defense against bacterial infection and virus.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/Pertanika%20PAPERS...

Abstract

The aims of the present study are to isolate and characterise structure TLR3 and IL1 gene and evaluate the potential and signaling mechanism following exposure of Polyinosinic: polycytidylic acid (poly (I:C)) Lipopolysaccharide (LPS) and Zymosan as antigens in gouramy (Osphronemus gouramy). Gouramy kidney cells were stimulated with LPS Poly I:C and Zymosan. Following incubation at 28C relative expression levels of Toll Like Receptor 3 (TLR3) and interleukin-1 (IL-1) were examined at one hour and six hour after treatment. A Real Time Polimerase Chain Reaction approach was utilised to search for the effects of Poly I:C LPS and Zymosan exposure to gouramy kidney cells between one hour to six hours after treatment LPS in kidney cell increased expression of interleukin-1 and downregulated expression of TLR3. Poly (I:C) which is an antigen that responds to antiviral induces an increase in the transcription both of Toll like receptor 3 and IL-1. Zymosan in kidney cells increased expression of TLR3 but downregulated expression of IL1. This study shows that TLR3 was activated not only by Poly I: C but also LPS and Zymosan. However antigen Poly IC-induced labor IL-1 and TLR3 expressed higher than the antigen LPS and Zymosan so this research showed a TLR3 response to Poly I:C is more dominant than Zymosan and LPS. TLR3 in gouramy kidney cell transcript was identified in response to poly I:C Zymosan and LPS. These results indicate that TLR3 in gouramy also plays a role for defense against bacterial infection and virus.

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Item Type:	Article
AGROVOC Term:	Osphronemus goramy
AGROVOC Term:	Kidneys
AGROVOC Term:	Cells
AGROVOC Term:	Lipopolysaccharides
AGROVOC Term:	Zymosan
AGROVOC Term:	Incubation of microorganisms
AGROVOC Term:	Cell culture
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Antigens
AGROVOC Term:	Bacteria control (disinfection)
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24830

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