Citation
Musthapa Iqbal, . and Gumilar Gun Gun, . and Dara Fitri, . Isolation of metyhl- piperate from n-hexane extract of fruit of Cabe Jawa (Piper retrofractum Vahl.). pp. 1489-1495. ISSN 1511-3701
Abstract
Cabe Jawa (Piper retrofractum Vahl.) is categorised in Pipers genus under family piperaceae. It is commonly reported to function as antimicrobial antifungal and anti-inflammatory. The aim of this study is to obtain information on the isolation of secondary metabolites from n-hexane extract of Cabe Jawa fruits. The isolation stage began with the maceration method with n-hexane solvent then continued with purification stage of fractination compounds which was conducted by using several chromatography techniques including thin layer chromatography (TLC) vacuum liquid chromatography (VLC) and radial chromatography. Methyl piperate compound was isolated from this extract. The structure of this compound was determined using spectroscopic 1H NMR. Based on the results of spectroscopic analysis of 1H NMR was present at a chemical shift of 3.80 ppm singlet peak with integrity of 3H was the typical peak of a methoxy. At 6.12 ppm chemical shift indicated a signal peak for protons bound to C sp2. Chemical shift at 6.96 ppm to 7.13 ppm for proton groups was attached to benzene ring. From the results of 1H NMR spectrum assisted by biogenesis approach it is concluded that the purified compound successfully isolated was a methyl piperate compound.
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Abstract
Cabe Jawa (Piper retrofractum Vahl.) is categorised in Pipers genus under family piperaceae. It is commonly reported to function as antimicrobial antifungal and anti-inflammatory. The aim of this study is to obtain information on the isolation of secondary metabolites from n-hexane extract of Cabe Jawa fruits. The isolation stage began with the maceration method with n-hexane solvent then continued with purification stage of fractination compounds which was conducted by using several chromatography techniques including thin layer chromatography (TLC) vacuum liquid chromatography (VLC) and radial chromatography. Methyl piperate compound was isolated from this extract. The structure of this compound was determined using spectroscopic 1H NMR. Based on the results of spectroscopic analysis of 1H NMR was present at a chemical shift of 3.80 ppm singlet peak with integrity of 3H was the typical peak of a methoxy. At 6.12 ppm chemical shift indicated a signal peak for protons bound to C sp2. Chemical shift at 6.96 ppm to 7.13 ppm for proton groups was attached to benzene ring. From the results of 1H NMR spectrum assisted by biogenesis approach it is concluded that the purified compound successfully isolated was a methyl piperate compound.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Piper retrofractum |
| AGROVOC Term: | Piperaceae |
| AGROVOC Term: | Isolation techniques |
| AGROVOC Term: | Macerating |
| AGROVOC Term: | Purification |
| AGROVOC Term: | Extracts |
| AGROVOC Term: | Chromatography |
| AGROVOC Term: | Thin layer chromatography |
| AGROVOC Term: | Spectroscopy |
| AGROVOC Term: | antimicrobial agents |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24831 |
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