Use of bio-chemical surfactant producing endophytic bacteria isolated from rice root for heavy metal bioremediation

Karnwal Arun, . Use of bio-chemical surfactant producing endophytic bacteria isolated from rice root for heavy metal bioremediation. pp. 699-714. ISSN 1511-3701

A variety of microorganisms generate highly potent surface-active bio-molecules or biosurfactants which vary in their chemical properties and molecular size. In the present study bioremediation effect of Pseudomonas fluorescence RE1 (GenBank: MF102882.1) and RE17 (GenBank: MF103672.1) endophytes on heavy metals Zn Cr Cd and Ni were investigated. A total of 56 morphologically distinct isolates from indigenous rice roots were selected and subsequently characterised genotypically by using 16S rRNA sequencing approach. Next biosurfactant production and heavy metal removal ability by the isolates were screened on the basis of ‚ and ‚ hemolysis on blood agar plates BATH assay and CTAB method. Analysis of bioremediation of heavy metals was done by using atomic adsorption spectroscopy. Bioremediation analysis revealed that isolates RE1 and RE17 reduced the concentration of Zn by up to 92 and 90 at pH 7.5 respectively while for Ni removal was the same for both strains at 95 at pH 7.5. Biosorption results for Cr and Cd showed highest metal removal efficiency by Pseudomonas fluorescence RE17 at pH 8 92 and 98 respectively. Both isolates showed significant metal removal efficiency at 32‚1‚C for all experimental heavy metals. The present study suggests that all endophytes withstand at high concentration of testing heavy metals and can be used for bioremediation of heavy metals in contaminated environments.

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Abstract

A variety of microorganisms generate highly potent surface-active bio-molecules or biosurfactants which vary in their chemical properties and molecular size. In the present study bioremediation effect of Pseudomonas fluorescence RE1 (GenBank: MF102882.1) and RE17 (GenBank: MF103672.1) endophytes on heavy metals Zn Cr Cd and Ni were investigated. A total of 56 morphologically distinct isolates from indigenous rice roots were selected and subsequently characterised genotypically by using 16S rRNA sequencing approach. Next biosurfactant production and heavy metal removal ability by the isolates were screened on the basis of ‚ and ‚ hemolysis on blood agar plates BATH assay and CTAB method. Analysis of bioremediation of heavy metals was done by using atomic adsorption spectroscopy. Bioremediation analysis revealed that isolates RE1 and RE17 reduced the concentration of Zn by up to 92 and 90 at pH 7.5 respectively while for Ni removal was the same for both strains at 95 at pH 7.5. Biosorption results for Cr and Cd showed highest metal removal efficiency by Pseudomonas fluorescence RE17 at pH 8 92 and 98 respectively. Both isolates showed significant metal removal efficiency at 32‚1‚C for all experimental heavy metals. The present study suggests that all endophytes withstand at high concentration of testing heavy metals and can be used for bioremediation of heavy metals in contaminated environments.

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Item Type:	Article
AGROVOC Term:	Oryza sativa
AGROVOC Term:	Rice
AGROVOC Term:	Roots
AGROVOC Term:	Heavy metals
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	RNA sequence
AGROVOC Term:	Bioremediation
AGROVOC Term:	Atomic absorption spectrometry
AGROVOC Term:	Biosorption (bioremediation)
AGROVOC Term:	Pseudomonas fluorescens
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24845

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