Citation
Ariff O., . and Wahid H., . and Yimer N., . and Baiee F. H., . and Rosnina Y., . Sperm DNA impairment in the bull: causes influences on reproduction and evaluations. pp. 63-80. ISSN 1511-3701
Abstract
Conventional semen examination involving sperm motility viability and morphology remains the backbone of assessing the fertility status of a sire. However there remains instances where these semen parameters appear normal but cases of low conception rates or failure of pregnancy occur. This review highlights the causes of sperm DNA damage and the effectiveness of techniques designed to evaluate the contribution of sperm DNA damage to lowered fertility in bulls. Among the many causes of sperm DNA impairment are imperfect spermatogenesis faulty apoptosis reactive oxygen species in-vitro handling impact of environment radiography and the stress of cryopreservation processes. Furthermore DNA impairment impairs fertilisation interferes with embryonic development and implantation and blocks blastocyst formation. The most frequently used tests to determine DNA damage are the acridine orange test (AOT) using acridine orange stain with examination under a fluorescence microscope and the sperm chromatin structure assay (SCSA) using the same stain but examined with flow cytometry.
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Abstract
Conventional semen examination involving sperm motility viability and morphology remains the backbone of assessing the fertility status of a sire. However there remains instances where these semen parameters appear normal but cases of low conception rates or failure of pregnancy occur. This review highlights the causes of sperm DNA damage and the effectiveness of techniques designed to evaluate the contribution of sperm DNA damage to lowered fertility in bulls. Among the many causes of sperm DNA impairment are imperfect spermatogenesis faulty apoptosis reactive oxygen species in-vitro handling impact of environment radiography and the stress of cryopreservation processes. Furthermore DNA impairment impairs fertilisation interferes with embryonic development and implantation and blocks blastocyst formation. The most frequently used tests to determine DNA damage are the acridine orange test (AOT) using acridine orange stain with examination under a fluorescence microscope and the sperm chromatin structure assay (SCSA) using the same stain but examined with flow cytometry.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Bulls |
AGROVOC Term: | Sperm |
AGROVOC Term: | DNA |
AGROVOC Term: | Semen |
AGROVOC Term: | Motility |
AGROVOC Term: | Viability |
AGROVOC Term: | morphology |
AGROVOC Term: | Fertility |
AGROVOC Term: | Cryopreservation |
AGROVOC Term: | Spermatogenesis |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 06:29 |
URI: | http://webagris.upm.edu.my/id/eprint/24853 |
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