Citation
Muhammad Fadzly Salleh, . and Ng Wei Lun, . and Ky Huynh, . and Noorjahan Banu Mohammed Alitheen, . and Siew Ging Yang, . and Tan Soon Guan, . and Tan Sheau Wei, . and Yeap Swee Keong, . Assessment of the genetic variation of Malaysian durian varieties using inter-simple sequence repeat markers and chloroplast DNA sequences. pp. 321-332. ISSN 1511-3701
Abstract
To date 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However the levels and patterns of genetic variation among the varieties are still unknown. In this study the leaves of 27 durian varieties were sampled from four durian orchards in Universiti Putra Malaysia namely Bukit Ekspo Putra Mart Ladang Puchong and Ladang 5. Twenty five inter-simple sequence repeat (ISSR) primers were tested for PCR amplification on DNA samples. Twelve ISSR primers amplified 133 clear and reproducible DNA fragments and 122 (91.73) were polymorphic indicating a high level of genetic variation among these durian varieties. Primers flanking four chloroplast DNA (cpDNA) regions (trnL-trnF atpB-rbcL and trnH-psbA intergenic spacers as well as the partial matK gene) were tested for PCR amplification. Two cpDNA regions (trnL-trnF and matK) were successfully amplified but showed no variation in their DNA sequences even when additional samples from Vietnam were included. The findings in this preliminary study lay a foundation for more comprehensive future studies on the genetic variation among durian varieties.
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Abstract
To date 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However the levels and patterns of genetic variation among the varieties are still unknown. In this study the leaves of 27 durian varieties were sampled from four durian orchards in Universiti Putra Malaysia namely Bukit Ekspo Putra Mart Ladang Puchong and Ladang 5. Twenty five inter-simple sequence repeat (ISSR) primers were tested for PCR amplification on DNA samples. Twelve ISSR primers amplified 133 clear and reproducible DNA fragments and 122 (91.73) were polymorphic indicating a high level of genetic variation among these durian varieties. Primers flanking four chloroplast DNA (cpDNA) regions (trnL-trnF atpB-rbcL and trnH-psbA intergenic spacers as well as the partial matK gene) were tested for PCR amplification. Two cpDNA regions (trnL-trnF and matK) were successfully amplified but showed no variation in their DNA sequences even when additional samples from Vietnam were included. The findings in this preliminary study lay a foundation for more comprehensive future studies on the genetic variation among durian varieties.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Durian |
| AGROVOC Term: | Durio zibethinus |
| AGROVOC Term: | Genetic diversity within species |
| AGROVOC Term: | Orchards |
| AGROVOC Term: | DNA sequence |
| AGROVOC Term: | Phenotypes |
| AGROVOC Term: | Chloroplasts |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Purification |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24870 |
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