Effects of solvent system drying and storage on the total phenolic content and antioxidant activities of Clinacanthus nutans Lindau (Sabah Snake Grass)

Chee K. S., . and Yu Y. C., . Effects of solvent system drying and storage on the total phenolic content and antioxidant activities of Clinacanthus nutans Lindau (Sabah Snake Grass). pp. 1685-1701. ISSN 1511-3701

The effects of extraction solvent system (boiling water water at room temperature 50 aqueous methanol and 100 methanol) drying (oven drying and sun drying) and storage (refrigeration) on the total phenolic content (TPC) and antioxidant activities of the extracts from Clinacanthus nutans Lindau (Sabah Snake Grass) leaves were studied. TPC was determined using Folin-Ciocalteau method. Antioxidant activities were evaluated using three different methods namely i) 2 2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay ii) ferric reducing antioxidant power (FRAP) assay and iii) beta-carotene bleaching (BCB) assay. Boiling water extraction of fresh leaves resulted in the highest TPC and DPPH and FRAP activities. However methanolic (100 methanol) extract from the fresh leaves showed the highest antioxidant activity in BCB. Both oven drying and sun drying caused a significant decrease in the antioxidant capacity with sun drying resulting in a lower TPC than oven drying. Cold storage (at 4 2 C) for three weeks after drying resulted in a decrease in TPC. A significant decrease was observed in DPPH FRAP and BCB activities after three weeks of refrigeration storage. TPC was strongly correlated to DPPH 1/EC50 (r 0.969 p 0.05) and FRAP (r 0.991 p 0.01) activities. However there was no correlation between TPC and BCB. In conclusion boiling water extraction of fresh C. nutans leaves resulted in the highest TPC and antioxidant activities. Drying and storage resulted in deterioration of the TPC and antioxidant activities of C. nutans leaves.

Full text available from: Official URL: http://www.pertanika2.upm.edu.my/Pertanika%20PAPER...

Abstract

The effects of extraction solvent system (boiling water water at room temperature 50 aqueous methanol and 100 methanol) drying (oven drying and sun drying) and storage (refrigeration) on the total phenolic content (TPC) and antioxidant activities of the extracts from Clinacanthus nutans Lindau (Sabah Snake Grass) leaves were studied. TPC was determined using Folin-Ciocalteau method. Antioxidant activities were evaluated using three different methods namely i) 2 2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay ii) ferric reducing antioxidant power (FRAP) assay and iii) beta-carotene bleaching (BCB) assay. Boiling water extraction of fresh leaves resulted in the highest TPC and DPPH and FRAP activities. However methanolic (100 methanol) extract from the fresh leaves showed the highest antioxidant activity in BCB. Both oven drying and sun drying caused a significant decrease in the antioxidant capacity with sun drying resulting in a lower TPC than oven drying. Cold storage (at 4 2 C) for three weeks after drying resulted in a decrease in TPC. A significant decrease was observed in DPPH FRAP and BCB activities after three weeks of refrigeration storage. TPC was strongly correlated to DPPH 1/EC50 (r 0.969 p 0.05) and FRAP (r 0.991 p 0.01) activities. However there was no correlation between TPC and BCB. In conclusion boiling water extraction of fresh C. nutans leaves resulted in the highest TPC and antioxidant activities. Drying and storage resulted in deterioration of the TPC and antioxidant activities of C. nutans leaves.

[error in script]

Item Type:	Article
AGROVOC Term:	Acanthaceae
AGROVOC Term:	Elephant grass
AGROVOC Term:	Leaves
AGROVOC Term:	Solvent extraction
AGROVOC Term:	Methanol
AGROVOC Term:	Sunlight
AGROVOC Term:	Ovens
AGROVOC Term:	Refrigerated storage
AGROVOC Term:	Drying
AGROVOC Term:	Boiling
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24886

Actions (login required)

View Item