In-vitro antioxidant potential in leaf stem and bark of Azadirachta indica

Abdulaziz Rabiu Abdulkadir, . and Nashriyah Mat, . and Md Sarwar Jahan, . In-vitro antioxidant potential in leaf stem and bark of Azadirachta indica. pp. 497-506. ISSN 1511-3701

Azadirachta indica (Meliaceae; Neem tree) is a resourceful medicinal plant. Almost all parts of the plant are used in traditional and folklore medicine. This study was conducted to measure antioxidant activity total phenolic content (TPC) and total flavonoid content (TFC) in a crude extract of different parts of the neem tree. 1 1-Diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging and Ferric-Reducing Antioxidant Power Assay (FRAP) were used to evaluate antioxidant activity while total phenolic and total flavonoid compounds were measured using the Folin-Ciocalteu method and aluminium chloride (AlCl3 ) assay respectively. Bark and leaf extract showed higher free radical scavenging activity with IC50 value of 23.27 and 55.07 (g/ml) respectively while seed extract showed the lowest activity with IC50 of 672.36 (g/ml; (P0.05). The bark extract revealed significantly higher antioxidant activity and phenolic content than the leaf and seed extract (P0.05). Flavonoid content in leaf extract was found to be significantly higher (P0.05) than in seed and bark extract; however bark extract showed significantly lower flavonoid content than seed extract. These results suggest that the potency of Azadirachta indica makes it a good source of natural antioxidant compounds.

Full text available from: Official URL: http://www.pertanika2.upm.edu.my/Pertanika%20PAPER...

Abstract

Azadirachta indica (Meliaceae; Neem tree) is a resourceful medicinal plant. Almost all parts of the plant are used in traditional and folklore medicine. This study was conducted to measure antioxidant activity total phenolic content (TPC) and total flavonoid content (TFC) in a crude extract of different parts of the neem tree. 1 1-Diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging and Ferric-Reducing Antioxidant Power Assay (FRAP) were used to evaluate antioxidant activity while total phenolic and total flavonoid compounds were measured using the Folin-Ciocalteu method and aluminium chloride (AlCl3 ) assay respectively. Bark and leaf extract showed higher free radical scavenging activity with IC50 value of 23.27 and 55.07 (g/ml) respectively while seed extract showed the lowest activity with IC50 of 672.36 (g/ml; (P0.05). The bark extract revealed significantly higher antioxidant activity and phenolic content than the leaf and seed extract (P0.05). Flavonoid content in leaf extract was found to be significantly higher (P0.05) than in seed and bark extract; however bark extract showed significantly lower flavonoid content than seed extract. These results suggest that the potency of Azadirachta indica makes it a good source of natural antioxidant compounds.

[error in script]

Item Type:	Article
AGROVOC Term:	Azadirachta indica
AGROVOC Term:	Meliaceae
AGROVOC Term:	Neem (tree)
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Leaf extracts
AGROVOC Term:	Stems
AGROVOC Term:	Bark extracts
AGROVOC Term:	Neem seed extract
AGROVOC Term:	Experiments
AGROVOC Term:	Phenolic content
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24908

Actions (login required)

View Item