Chromatographic profiling to determine the extent of structural changes in arrowroot starch (Marantha arundinacea L.) modification


Citation

Pratiwi M., . and Faridah D. N., . and Lioe H. N., . Chromatographic profiling to determine the extent of structural changes in arrowroot starch (Marantha arundinacea L.) modification. pp. 2519-2523. ISSN 2231-7546

Abstract

Arrowroot starch (Marantha arundinacea L.) has demonstrated to have a potential resistant starch type III (RS3). Certain techniques of starch modification could increase the yield of resistant starch. The effect of multiple treatments of acid hydrolysis debranching autoclavingcooling cycles and heat moisture treatment (HMT) towards the structural changes of arrowroot starch especially with HMT as the additional treatment was investigated in this study. This study revealed the molecular distribution profile of arrowroot starch after some modification which might be related to the starch resistance by using gel filtration chromatography (GFC). GFC profiles could distinguish the extent of starch structural changes shown by the different molecular distribution profile. In general modification by the multiple treatments led to the decrease of amylopectin and increase of amylose fractions. Amylose fraction has been known to contribute to a more rapid retrogradation process as a mechanism of resistant starch formation. Combination of acid hydrolysis debranching autoclaving-cooling and HMT treatments obviously altered the starch structures according to GFC profiles. The intensive degradation of amylopectin into amylose fragments could be determined. This fact might be associated to the starch resistance.


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Abstract

Arrowroot starch (Marantha arundinacea L.) has demonstrated to have a potential resistant starch type III (RS3). Certain techniques of starch modification could increase the yield of resistant starch. The effect of multiple treatments of acid hydrolysis debranching autoclavingcooling cycles and heat moisture treatment (HMT) towards the structural changes of arrowroot starch especially with HMT as the additional treatment was investigated in this study. This study revealed the molecular distribution profile of arrowroot starch after some modification which might be related to the starch resistance by using gel filtration chromatography (GFC). GFC profiles could distinguish the extent of starch structural changes shown by the different molecular distribution profile. In general modification by the multiple treatments led to the decrease of amylopectin and increase of amylose fractions. Amylose fraction has been known to contribute to a more rapid retrogradation process as a mechanism of resistant starch formation. Combination of acid hydrolysis debranching autoclaving-cooling and HMT treatments obviously altered the starch structures according to GFC profiles. The intensive degradation of amylopectin into amylose fragments could be determined. This fact might be associated to the starch resistance.

Additional Metadata

[error in script]
Item Type: Article
AGROVOC Term: Marantaceae
AGROVOC Term: Arrowroot
AGROVOC Term: Starch
AGROVOC Term: Hydrolysis
AGROVOC Term: Heat treatment
AGROVOC Term: Chromatography
AGROVOC Term: Gel filtration chromatography
AGROVOC Term: Amylopectin
AGROVOC Term: Amylose
AGROVOC Term: Structural change
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:29
URI: http://webagris.upm.edu.my/id/eprint/24950

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