Physicochemical characterization of enzymatically prepared fish protein hydrolysate from waste of shortfin scad (Decapterus macrosoma)


Citation

Norizah Mhd Sarbon, . and Nabilah Husna Ishak, . Physicochemical characterization of enzymatically prepared fish protein hydrolysate from waste of shortfin scad (Decapterus macrosoma). pp. 2593-2600. ISSN 2231-7546

Abstract

The aim of the present study is to report on the physicochemical characterization of shortfin scad (Decapterus macrosoma) waste hydrolysate (SWH) enzymatically prepared using alcalase. The characterization incorporates chemical composition (moisture protein fat ash) protein concentration molecular weight (SDS- PAGE) amino acid composition solubility and structure properties of shortfin scad waste hydrolysate (SWH) via Fourier transform infrared (FTIR) spectroscopy. SWH contains an average of 5.06 0.47 moisture 73.08 1.54 protein 7.55 0.90 fat and 10.40 0.13 ash with a high protein concentration (30.80mg/ ml). The SDS-PAGE result showed that molecular weight of SWH was less than 17kDa. The amino acid composition of SWH was found to be high in glutamic acid/glutamine (12.39 0.59) and aspartic acid/asparagine (7.89 0.18) followed by glycine (7.15 0.39) lysine (6.80 0.15) arginine (6.38 0.08) and leucine (5.99 0.10). Fourier transform infrared (FTIR) spectra showed that SWH presented a similar structure to that shortfin scad waste (SW). In addition protein solubility in SWH increased to 92.98 by increasing pH level (pH 4 to pH 10). These findings demonstrate the promising potential of shortfin scad waste hydrolysate for the application as natural bioactive sources due to high protein content and concentration lower molecular weight high solubility and high percentage of essential amino acids which fulfil adult human requirements.


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Abstract

The aim of the present study is to report on the physicochemical characterization of shortfin scad (Decapterus macrosoma) waste hydrolysate (SWH) enzymatically prepared using alcalase. The characterization incorporates chemical composition (moisture protein fat ash) protein concentration molecular weight (SDS- PAGE) amino acid composition solubility and structure properties of shortfin scad waste hydrolysate (SWH) via Fourier transform infrared (FTIR) spectroscopy. SWH contains an average of 5.06 0.47 moisture 73.08 1.54 protein 7.55 0.90 fat and 10.40 0.13 ash with a high protein concentration (30.80mg/ ml). The SDS-PAGE result showed that molecular weight of SWH was less than 17kDa. The amino acid composition of SWH was found to be high in glutamic acid/glutamine (12.39 0.59) and aspartic acid/asparagine (7.89 0.18) followed by glycine (7.15 0.39) lysine (6.80 0.15) arginine (6.38 0.08) and leucine (5.99 0.10). Fourier transform infrared (FTIR) spectra showed that SWH presented a similar structure to that shortfin scad waste (SW). In addition protein solubility in SWH increased to 92.98 by increasing pH level (pH 4 to pH 10). These findings demonstrate the promising potential of shortfin scad waste hydrolysate for the application as natural bioactive sources due to high protein content and concentration lower molecular weight high solubility and high percentage of essential amino acids which fulfil adult human requirements.

Additional Metadata

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Item Type: Article
AGROVOC Term: Decapterus macrosoma
AGROVOC Term: Scad
AGROVOC Term: Fpc (fish protein concentrate)
AGROVOC Term: Physicochemical properties
AGROVOC Term: Protein hydrolysate
AGROVOC Term: Chemical composition
AGROVOC Term: Moisture content
AGROVOC Term: Protein content
AGROVOC Term: Fat content
AGROVOC Term: Ash content
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:29
URI: http://webagris.upm.edu.my/id/eprint/24958

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